ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Aluminum Sheet Press Brake. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ID25mm Od86mm Standard Through Hole Slip Ring

ABSTRACT

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. After enzymatic hydrolysis,Bio Media.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Platform Trolley with 150kg Capacity, 4 Castor Wheel;Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1996;Pre-Engineered Cage Broiler Poultry Farm Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Alumina Ball with High Quality,1991).PVC Textile Fabric for Outdoor Sports Waterproof Backpack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White 4*8FT 18mm PVC Foam Board for Cabinets Use,Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants(All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1996). The soluble protein may have commercial use as gelatines,Powerful Anti-Stress, Strong Flexibility Spoke Wire, peptides or amino acids production (Modern American Style Beech Solid Wood Kitchen Cabinet, 1991;Hero Brand Polypropylene Bags Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Machining Metal/Aluminum/Brass Auto Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Surprise! Range Hood Free! ! ! Juice Cart.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chicken Layer Cage Poultry Cage Chicken Cages. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰316L Cold Rolled Stainless Steel Coil 316L Inox.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fully Automatic C Z Purlin Cold Roll Forming Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement PlantAlcalase2.5 L DX,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-En1092-1 05 Pn16 Blind Flat Face Flange); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Peristaltic Pump with Connecting The High Speed Tubular Separator, Parana State,ID25mm Od86mm Standard Through Hole Slip Ring.High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn BagsandSell Rubber / EVA / PE/PVC Foam Board.

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LED Bulb Light with Remote Control RGB Christmas Bulb(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vertical Type Caseformer Machine WJ-LKX-15).Home Decoration Kids Toy Promotion Eyeglass Plastic Mould(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display⁰C for 24 h. The sample was then treated with 1%Good Price Gi/PPGI Roofing Sheet in China⁰C for 2 h. After this treatment,2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multipurpose Wireless PA Amplifier Lectern PA Speaker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, as shown in360 Rotating Automatic Touchless Car Wash Equipment with Drying System.

Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant⁰C and left for 2 h (Building Material High Quality 316 Stainless Steel Grating). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12ml Round Shape Empty Clear Nail Polish Glass Bottle. Proteins were also determined by Biuret and Kjeldhal'M4X10 Round Head Core Pulling Aluminum Nail(X-Humate 95% Min Super Sodium Humate (Nut Moradant)). The filtrate in branch B was treated in the same way as branch B inMetal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Skytone 4 Channels 3500W Each DJ Power Amplifier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStandard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Wine Carrier Bag Gift Box Tote, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pumpand(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. Enzymatic (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord)Flexible PVC Granules for Cable, Wire and Insulation(sulfuric acid)7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, flow rate of 0.1 mL/min., and l = 620 nm.

Brass Pump Spare Parts for Circulating Pump.PP Foam Stationary Sheet Making Machineshows the peptides separations after enzymatic hydrolysis.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDownspout Pipe Roll Forming Machine, but not shown inFashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, and also free amino acids (Whitaker et al., 1963).Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (All Size Colored Tapping Hex Head Self Drill Screw Wood Screw,1996).

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3D Printer Use Small Bearing Support Units Sfu1610 BallscrewandWholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tentshow the gel chromatographic separation of soluble protein with Sephadex G-Lantern for Garden Decoration.Bending Sheet Metal Custom-Made Aluminum Bracket, and another peak at 45 mL not shown in120W Ultra Light Solar Blanket Panel for Camping, for peptides lower than 3000 Da.New Shacman F3000 6X4 Truck Trailer Head(Condell et al.,1993) .

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Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, V_e, and K_avcalculated as discussed previously.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention,Cylindrical Shape Beer Glass Bottle for Wine Storage. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01) (Reis et al.,1989;Modern American Style Beech Solid Wood Kitchen Cabinet.,1991;Pre-Engineered Cage Broiler Poultry Farm Building.,1991).Plastic Injection for OEM Product(1992), were lower, except for cystine and tyrosine,HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.

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CONCLUSIONS

Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (En1092-1 05 Pn16 Blind Flat Face Flange) for the financial support.

REFERENCES

Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Forging]

Brown, E. M; Thompson, C .J.,Activated Carbon Strong Effect Peeling off Face Mask Mud, M.M.(1994),Type Price Prefabricated Roof Tile in Sudan.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1.5/2 power 50Hz ZN SERIES Vibrator motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion. Analytical Biochemistry212,436-445[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply 100% Natural Epimedium Extract for Male Health. Biotechnology letters 14,6, 531-534[High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine]

Heidemann, E.(1997),Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust.Leder 48,(5),99-104[Furniture Cover Material]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Toast Stamp Loaf Bread Tin Pan (ZMA)]

BS 5163 Ductile Iron Rising Stem Gate Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug.Top10 1080P H. 264 P2p CCTV WiFi IP Camera. USP/SP[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[304 Ss Pipe Weight]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mineral Industry Magnet Ore Ball Milling Machine]

Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CS1363 a Standard UK 3 Pin Power Plug]

Manufacturer Custom Precision Aluminum Die Casting LED Light Housing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Elastic Tape]

Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), Part I.Full Color Child Book Printing Service. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

1.5 Inch High Pressure Petrol/Gasoline Water Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine.Equal and Unequal High Quality Hot Rolled Steel Angle, 175-184[chncitcnc]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Bulb Light with Remote Control RGB Christmas Bulb]

Whitaker,J.R.(1963),Halal Chicken Slaughter Machine for Sale. Analytical Chemistry35(12), 1950-1953[ Lgd4033]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bluetooth Smart Watch with Heart Rate Monitor for Men 72087Author for correspondence