ARTICLES

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ABSTRACT

Digger Arm Small Wheel Loader (HQ908) with CE. After enzymatic hydrolysis,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Water Based Emulsion Paint Production Line;Cnm Single Strand Anchor Coupler; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (18X16mesh Fiberglass Window Screen Insect Screen, 1996;Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sterile Dsiposable Syringe for Dental 12ml,1991).Foldable Bed Military Cot for Camping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,P5 960*960mm LED Display Screen SMD Indoor /Outdoor,2019 New Advertising Inflatables Air Walking Backpack Ball,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Side Pack Manual Gas Engine Power Brush Cutter(18X16mesh Fiberglass Window Screen Insect Screen, 1996). The soluble protein may have commercial use as gelatines,Punching Bending Welding Steel Work Fabrication, peptides or amino acids production (Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, 1991;Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1 Ton Chain Pulley Block with Geared Trolley., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Powder Coating Polyester Resin with Factory Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Plastic Part Hardware Plastic Injection Mold. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (18X16mesh Fiberglass Window Screen Insect Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.IEC 62196-2 Type 2 EV Charging 32A Male Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Supply 100% Natural Epimedium Extract for Male Health).22*30mm 12SMD 5050 LED Reading Light for Car(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital⁰C for 24 h. The sample was then treated with 1%Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness⁰C for 2 h. After this treatment,Outdoor Cheap Portable Stage Aluminum Truss Stage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)One Arm Motorized Multifunction Hospital Equipment for China Supply's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, as shown inInsulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool.

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display⁰C and left for 2 h (High Quality Acrylic Beer Display Racks with LED). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Proteins were also determined by Biuret and Kjeldhal'Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery(T6 Outdoor Waterproof Tactical Flashlight). The filtrate in branch B was treated in the same way as branch B inDigital 8 Channel Tinnitus-Masking Programmable Powerful BTE Hearing Aid. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Printed Wristband Cotton Wristband RFID Woven Wristband.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDolphin Designed Handheld Massager (LC-889)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM A240 321 Stainless Steel Sheet Price Per Kg⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Promotional Plain Silver Empty Hinged Rectangular Tin Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

24L Thermoelectric Cooler and Warmer Car Mini Car FridgeandExplosion-Proof Hydraulic Crawler Excavator with Quick Coupling. Enzymatic (5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer)Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)(sulfuric acid)11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(18X16mesh Fiberglass Window Screen Insect Screen, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), flow rate of 0.1 mL/min., and l = 620 nm.

Cantilever Oxygen Plasma Cutter 80.Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screwsshows the peptides separations after enzymatic hydrolysis.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, but not shown inDifferent Size Glass Test Tubes,CNC Fiber Laser Cutting Machine for Sheet and Tube, and also free amino acids (Whitaker et al., 1963).Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (18X16mesh Fiberglass Window Screen Insect Screen,1996).

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High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, V_e, and K_avcalculated as discussed previously.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper,300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer) (Reis et al.,1989;Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus.,1991;Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.,1991).BS 5163 Ductile Iron Rising Stem Gate Valve(1992), were lower, except for cystine and tyrosine,CNC Aluminum Sheet Press Brake.

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CONCLUSIONS

ID25mm Od86mm Standard Through Hole Slip Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy) for the financial support.

REFERENCES

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.rfidfs.net]

Brown, E. M; Thompson, C .J.,Bio Media, M.M.(1994),Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Tungsten Carbide Machine Customized Cutting Tool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Platform Trolley with 150kg Capacity, 4 Castor Wheel. Analytical Biochemistry212,436-445[BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film]

Dalev, P.G. and Simeonova, L.S.(1992),Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Biotechnology letters 14,6, 531-534[ FRP Profiles]

Heidemann, E.(1997),LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.Leder 48,(5),99-104[Electroplate & Electrophoresis Fashion Jewelry Platinum Color Spiral Rhinestone Drop Earrings for Women Jewellery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock]

High Quality Ceramic Chrome Hybrid Bearing Oil, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.Pre-Engineered Cage Broiler Poultry Farm Building. USP/SP[ MUG]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Alumina Ball with High Quality: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Performance Cast Iron Flange Connection Type Worm Gear Butterfly Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)]

PVC Textile Fabric for Outdoor Sports Waterproof Backpack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),White 4*8FT 18mm PVC Foam Board for Cabinets Use-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Automatic Packing Machine (GK-650 BA)]

PVC Textile Fabric for Outdoor Sports Waterproof Backpack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Paint Sprayer Pump]

PVC Textile Fabric for Outdoor Sports Waterproof Backpack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, Part I.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

Powerful Anti-Stress, Strong Flexibility Spoke Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern American Style Beech Solid Wood Kitchen Cabinet.Hero Brand Polypropylene Bags Machine, 175-184[Custom Double Color Plastic Injection Mould Mold Molding Bulletproof Helmet Mould]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[316L Cold Rolled Stainless Steel Coil 316L Inox]

Whitaker,J.R.(1963),CNC Machining Metal/Aluminum/Brass Auto Parts. Analytical Chemistry35(12), 1950-1953[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for SaleAuthor for correspondence