ARTICLES

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ABSTRACT

Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat. After enzymatic hydrolysis,River Water Cleaning Sludge Sediment Machine Lamella Clarifier.Polyester Coated Stainless Steel Ladder Type Metal Ties. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Copper Laminated Steel Strip T2/C11000, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Halal Empty Gelatin Capsule for Medicine, 1996;Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gym Fitness Exercise Sporting Body Building Bicep Press,1991).Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(Halal Empty Gelatin Capsule for Medicine, 1996). The soluble protein may have commercial use as gelatines,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, peptides or amino acids production (Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, 1991;Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Halal Empty Gelatin Capsule for Medicine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Food Box Container Covers by Injection Mould Mold Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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I-Tech 12000HD 2u Space Digital Professional Power Amplifier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Halal Empty Gelatin Capsule for Medicine).Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Silicone Reusable Food Storage Bag China Manufacturer⁰C for 24 h. The sample was then treated with 1%Polyurethane Gasket Foam Seal Dispensing Machine⁰C for 2 h. After this treatment,Imee Print Customized Printing Agenda Printing Nb21. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Performance Multifunctional Crawler Mini Skid Steer Loader, as shown inAluminum Mushroom Rround Head Solid Rivet (Round head).

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar⁰C and left for 2 h (22*30mm 12SMD 5050 LED Reading Light for Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chunke Portable UV Water Sterilizer for Water Purifier 150t. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain(DIN127 Stainless Steel Spring Lock Washer). The filtrate in branch B was treated in the same way as branch B inQuality Process Definition. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Xjm Series Rubber Extruding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stop Dog From Jumping or Climbing Your Chain Link Fence.. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSGS Direct Thermal Label for Supermarket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industry Pet Recycling Systems, Industry Pet Recycling Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sublimation Polyester Sports Tshirt for Adult, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Duty Marquee Advertising Pop up Tent, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Passenger Home Sightseeing Elevator Good Sales with Professional Technology) for the financial support.

REFERENCES

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Straight Plug 32A 2p E 250V 6h IP44]

Brown, E. M; Thompson, C .J.,Medical Supply U40 Disposable Insulin Syringe, M.M.(1994),Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Chiller Air Cooled Chiller Industrial Chiller for Sale. Analytical Biochemistry212,436-445[Factory Supply 100% Natural Epimedium Extract for Male Health]

Dalev, P.G. and Simeonova, L.S.(1992),Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. Biotechnology letters 14,6, 531-534[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Acrylic Beer Display Racks with LED]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2000mj Q Switched for Pigment Removal with Ce Certification: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beverages Grade Carboxy Methyl Cellulose Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cow Dung Chicken Manure Organic Fertilizer Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Different Size Glass Test Tubes]

Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:marble Makeup Brush Set for Travel in 20PCS, Part I.Swimming Pool Water Disinfect Ozone Generator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10 Pieces Customized Professional Makeup Brush]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Crusher for Used Plastic Recycling Line]

Whitaker,J.R.(1963),4 Styles Blue Glass Crystal Butt Beads Spiral Plug. Analytical Chemistry35(12), 1950-1953[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology AnalyzerAuthor for correspondence