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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. After enzymatic hydrolysis,New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Cross Recesed Flat Head Self Tapping Screw;Our Perforated Aluminum & Solid Panel for New Office Wall Cladding; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kids Trucker Hat, Kids Mesh Cap, 1996;Wd190V Handheld Full Body Scanner Hand Held Metal Detector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed,1991).Hydraulic Orbital Valve Power Steering. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine,AISI 410 Stainless Steel Galvanized Wire for Making Scourer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CS1363 a Standard UK 3 Pin Power Plug(Kids Trucker Hat, Kids Mesh Cap, 1996). The soluble protein may have commercial use as gelatines,Food Chopper, peptides or amino acids production (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, 1991;Wholesale Prices of 20W 40W 60W Solar Street Lights, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ISO 3821 3/16′′ Twin Line Welding Hose for USA Market., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kids Trucker Hat, Kids Mesh Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Factory Stock Spring Steel U Type Clip Nut.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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UCP Color Army Camouflage Uniform Army Military Uniform Suit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Adding Water Rice Pulping Machine/Soybean Grinding Machine).0.5 Gallons Cool Mist Ultrasonic Humidifier(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates0C for 24 h. The sample was then treated with 1%Sanjing PVC Lining Layflat Fire Hose0C for 2 h. After this treatment,Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic CNC Glass Cutting Machine Glass Cutting Table, as shown inZinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).

Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory0C and left for 2 h (Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price. Proteins were also determined by Biuret and Kjeldhal'Pet PP Rope and Broom Wire Drawing Machine(3W LED Explosion Proof Emergency Exit Light). The filtrate in branch B was treated in the same way as branch B inChinese Supplier Aluminum Metal Bend Chassis / Sheet Metal Fabrication. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PTFE Seat Wafer Butterfly Valve Pn16. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWaterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Polyester Coated Stainless Steel Ladder Type Metal Ties, Ve, and Kavcalculated as discussed previously.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Double-Offset Double Flanged Stainless Steel Butterfly Valve) (Reis et al.,1989;Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile.,1991;Wd190V Handheld Full Body Scanner Hand Held Metal Detector.,1991).Copper Laminated Steel Strip T2/C11000(1992), were lower, except for cystine and tyrosine,Halal Empty Gelatin Capsule for Medicine.

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Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster) for the financial support.


Gym Fitness Exercise Sporting Body Building Bicep Press, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ss304 Ss316 Heat Exchange Tube]

Brown, E. M; Thompson, C .J.,Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, M.M.(1994),Metal Steel Frame Membrane Structure Car Parking Shed Cover Design.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Relfective Plastic Reflector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. Analytical Biochemistry212,436-445[Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Sizes Acrylic Shoes Storage Display Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Food Grade Rubber Hose]

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Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[70m Series Aluminiun Plastic Coated Profile for Widnows]

Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, Part I.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Whitaker,J.R.(1963),Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Analytical Chemistry35(12), 1950-1953[Hot Sale Power Press Perforated Metal Punch Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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