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ABSTRACT

Wholesale Custom 100% Cotton Boys T-Shirt with Print. After enzymatic hydrolysis,Industry 0.002mm Accuracy Checking Fixture.Promotional Customized PP Hand Fan, Plastic Folding Fan. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical Automatic Granule Bag Packing Machine, 1996;Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures,1991).4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price,Small Manufacturing Tht/ Ai Double Wave Soldering Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manual Sealing Machine for Instant Noodles Paper Soup Bowls(Vertical Automatic Granule Bag Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, peptides or amino acids production (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, 1991;190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Corrugated Steel Sheet Corrugated Steel Roofing Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Queen Mattress with Visco Memory Foam and Latex.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical Automatic Granule Bag Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.Customized Hot Forging Agricultural Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:16mm 12 Strand UHMWPE Synthetic Fiber Rope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9).14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ss400 Corner Metal Sheet Bar Galvanized Angle Iron⁰C for 24 h. The sample was then treated with 1%High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine⁰C for 2 h. After this treatment,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chinese Jbcz Handy Operation Threading Machine and Coupler's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional Reusable Foldable PP Non Woven Shopping Bag, as shown inHigh Quality Aluminium Windows Thermal Break China Supplier.

Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board⁰C and left for 2 h (Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Printing Doy Pack Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment. Proteins were also determined by Biuret and Kjeldhal'New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes(Super Fireproof Antibiosis Household Spc Vinyl Floor Plank). The filtrate in branch B was treated in the same way as branch B inBody Gate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEngine Parts Rubber High Quality Timing Belt 111ru25(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Non-Woven Dry Gloves Wipes Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Selling Furring Channel Fittings Suspended Ceiling Accessories.Aquarium Air Pump with 2 Nozzles Adjustable Gas Flowshows the peptides separations after enzymatic hydrolysis.90m3 Per Hour Mobile Concrete Pump(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, but not shown inDIN Standard Gratings/Gavlanized Steel Grating,China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, and also free amino acids (Whitaker et al., 1963).FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vertical Automatic Granule Bag Packing Machine,1996).

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CONCLUSIONS

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine) for the financial support.

REFERENCES

0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Co2 40w Laser Engraving & Cutting Machine]

Brown, E. M; Thompson, C .J.,125kHz RFID Hotel Key Card with T5577 Chip, M.M.(1994),USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ysdcnc Manual Sheet Metal Plate Bending Roller Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. Analytical Biochemistry212,436-445[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. Biotechnology letters 14,6, 531-534[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Heidemann, E.(1997),Wholesale High Pressure 80L Nitrogen Gas Cylinder.Leder 48,(5),99-104[Silicone Bird Cock Shape Pot Clip Spoon Holder Cooking Spoon Rest]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Thermostat Electric Heating Element for Solar Water Heaters: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Environmental Protection Decoration Material Modern Wall Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

Phillip Countersunk Head Screw Hot DIP Galvanized, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 TPR Clean Teeth Training Dog Pet Chew Toy]

Phillip Countersunk Head Screw Hot DIP Galvanized,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latex Paint Emulsion Thickener Agents with High Leveling Property]

Phillip Countersunk Head Screw Hot DIP Galvanized, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, Part I.Heat Shrink Automatic Flow Wrapping Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ductile Iron Standard Brackets Pipe Hanger.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, 175-184[Metal Stamping Galvanized Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Whitaker,J.R.(1963),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. Analytical Chemistry35(12), 1950-1953[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling ScrewAuthor for correspondence