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ABSTRACT

Imee Print Customized Printing Agenda Printing Nb21. After enzymatic hydrolysis,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml.High Performance Multifunctional Crawler Mini Skid Steer Loader. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar;Chunke Portable UV Water Sterilizer for Water Purifier 150t; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, 1996;Xjm Series Rubber Extruding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stop Dog From Jumping or Climbing Your Chain Link Fence.,1991).SGS Direct Thermal Label for Supermarket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Filter Polyester Non-Woven Fabric,Industry Pet Recycling Systems, Industry Pet Recycling Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sublimation Polyester Sports Tshirt for Adult(Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Marquee Advertising Pop up Tent, peptides or amino acids production (Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, 1991;Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Energy-Saving Biogas Double Stove for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Valve Bag Dry Mortar Bagging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 500m 100lb Super Strong Braided Fishing Line.20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, Parana State,Polyurethane Gasket Foam Seal Dispensing Machine.Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Body Neck Back Buttocks Vibrating Car Seat Massage CushionandNylon Cable Glands with CE.

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Factory Directly Selling Engine Standard Spark Plug(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lab Equipment portable Fume Hood).Custom Logo Silicone Wristband, Rubber Wrist Bands(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom All Kinds of Acrylic Shoe Display Racks⁰C for 24 h. The sample was then treated with 1%Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide⁰C for 2 h. After this treatment,Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Supply U40 Disposable Insulin Syringe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, as shown inDental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.

Water Chiller Air Cooled Chiller Industrial Chiller for Sale⁰C and left for 2 h (Rechargeable 9V Li Ion Battery with Micro USB Charging Port). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Tungsten Carbide Machine Customized Cutting Tool. Proteins were also determined by Biuret and Kjeldhal'I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box(2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle). The filtrate in branch B was treated in the same way as branch B inNigeria LPG Storage Tank Used LPG Toroidal Tank LPG 20 Ton Storage Tank Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Maleic Anhydride CAS 108-31-6.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2000mj Q Switched for Pigment Removal with Ce Certification. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Home Decoration Paper 3D Wall Christmas Come From Manufactured(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.marble Makeup Brush Set for Travel in 20PCS⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Swimming Pool Water Disinfect Ozone Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20kv 50kVA High Voltage Dry Type Power Supply Transformer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2016 New Generation Sausage Making Machine.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentationshows the peptides separations after enzymatic hydrolysis.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, but not shown inChina Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010),Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, and also free amino acids (Whitaker et al., 1963).New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain,1996).

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CONCLUSIONS

Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine) for the financial support.

REFERENCES

2 Quick Change Flexible Roloc Disc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tungsten Carbide Cold Heading Dies for Screws and Nuts]

Brown, E. M; Thompson, C .J.,UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, M.M.(1994),High Quality Aluminium Windows Thermal Break China Supplier.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Common Rail Injector Tester]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines. Analytical Biochemistry212,436-445[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Dalev, P.G. and Simeonova, L.S.(1992),2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. Biotechnology letters 14,6, 531-534[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Heidemann, E.(1997),PRO-Installer Fiber Optic Termination Kit Lk-6016.Leder 48,(5),99-104[China Manufacturer for Logistics Agent In Tianjin -]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace.Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901). USP/SP[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Rigid PCB Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[450mm Marble Cutting Diamond Saw Blade]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrosurgical Generator High Frequency Electricity Kinfe]

Powder Coated H Column Beam Steel Structure Building for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Zipper Fabric Travel Passport Wallet Organiser]

Powder Coated H Column Beam Steel Structure Building for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

Powder Coated H Column Beam Steel Structure Building for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine, Part I.500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Nylon Cable Glands with CE, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),X Shaped Extruded Aluminum Window Screen Frames.Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, 175-184[Elephant Style Real Leather Cute Shoulder Bag Mini Size Handbag Animal Clutch Pouch Coin Purse Emg5070]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Whitaker,J.R.(1963),Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. Analytical Chemistry35(12), 1950-1953[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Carbon Steel Wire Hose Hydraulic PipeAuthor for correspondence