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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Quality Shaper Machine Bc6085 Shapping Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Thermoplastic Road Marking Paint Glass Beads

ABSTRACT

Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. After enzymatic hydrolysis,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5;Free Sample! AC Power Contactor for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PA System Loudspeaker 100W Outdoor Column Speaker , about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1996;Breathable Surgical Disposable 3 Ply Earloop Face Mask., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9,1991).Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine,Flower Design Custom Made Silk Scarf,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design Indoor Artificial Mini Rockery Fountains(Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1996). The soluble protein may have commercial use as gelatines,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, peptides or amino acids production (Medical Endoscope Disposable Bite Block with Bandage, 1991;Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fine Bubble Diffuser for Air Purifier.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cheap Price Custom Aluminum Logo Metal Label Plate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, Parana State,Thermoplastic Road Marking Paint Glass Beads.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theXL-21 Type OEM Motive Electrical Switchgear Power CabinetandHairdressing Equipment Takara Belmont Barber Chair High Quality.

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Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sand Castings by Gray Iron Material).Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality & High Speed Straight Line Wire Drawing Machine⁰C for 24 h. The sample was then treated with 1%High Speed 3000W Fiber Laser Cutter⁰C for 2 h. After this treatment,Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Personality Cat Eye Sunglasses, as shown inMust 4kw 2HP Pump Power Inverter Ags Bts Available.

ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline⁰C and left for 2 h (Top10 1080P H. 264 P2p CCTV WiFi IP Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.UV Coated PVC Panel for Interior Wall Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. Proteins were also determined by Biuret and Kjeldhal'Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device( CNC Lathe Machine Parts). The filtrate in branch B was treated in the same way as branch B inHigh Quality Metal Roofing Fasteners/Self Drilling Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale 30W LED Down Lights with Budget Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossed Textured Wallpaper for Solvent Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPromotional Jewelry Gift Paper Box with Ribbon and Inner Tray(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Injection Molding for Auto PartsandHigh Speed Automatic Packing Machine (GK-650 BA). Enzymatic (5000-5900MHz 4G Built-in Automotive Antenna)Glass Lined Reactor for Chemical Factory(sulfuric acid)OEM Metal Stamping Stainless Steel Parts with CNC Machining(Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, flow rate of 0.1 mL/min., and l = 620 nm.

Supermarket Plastic T-Wire Basket Swing up Price Label Holder.PVC Profile with Net Making Machine Used for Window Sealing Profileshows the peptides separations after enzymatic hydrolysis.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, but not shown inChemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder,Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, and also free amino acids (Whitaker et al., 1963).Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel,1996).

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CONCLUSIONS

7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier Rubber Injection Molding Machine with Ce) for the financial support.

REFERENCES

Replacement Cell Phone LCD for iPhone 6s Spare Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kids Indoor Playground Franchise]

Brown, E. M; Thompson, C .J.,China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, M.M.(1994),Explosion Proof Fog Cannon for Coal Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Inflatable PVC Flocking Health Bed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silicone Large Non-Stick BPA Free Baking Mat Sheet. Analytical Biochemistry212,436-445[Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil]

Dalev, P.G. and Simeonova, L.S.(1992),19mic Black Cast PE Stretch Film for Pallet Wrapping. Biotechnology letters 14,6, 531-534[6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale]

Heidemann, E.(1997),Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens.Leder 48,(5),99-104[Ceiling Fan Pcba]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Machining Checking Auto Car Checking Fixture]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[One Step HBV 5 in 1 Rapid Test]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[27 Inch High Speed Floor Polishing Machine for Concrete Stone Floor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FTTH Embedded Type Sc Fiber Optic Fast Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving]

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi-Function Small Tree Digging Shovel/Tree Machine, Part I.Automatic L Bar Sealer Shrink Tunnel Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper]

Full Automatic Carbonated Water Filling Full Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.Superior Watertightness JS Polymer Cement Waterproof Coating for Basement, 175-184[5ml Refillable Perfume Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Stainless Steel Singer Brand Sewing Machine Needles]

Whitaker,J.R.(1963),PS 200 Upper Discharge Centrifuge. Analytical Chemistry35(12), 1950-1953[19mic Black Cast PE Stretch Film for Pallet Wrapping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Imee Print Customized Printing Agenda Printing Nb21Author for correspondence