ARTICLES

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ABSTRACT

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen. After enzymatic hydrolysis,OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP).Ms Gi Pipe Dn15~Dn400 Gi Pipe List. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low-E Insulating Glass Curtain Wall;Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Design Underwear for Men Cotton Mens Boxer Briefs, 1996;Nylon Balance Bike with Water Transfer Printing Malaysia., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti Slip Rubber Mat,1991).5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor,Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml(Fashion Design Underwear for Men Cotton Mens Boxer Briefs, 1996). The soluble protein may have commercial use as gelatines,Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, peptides or amino acids production (Automotive Perforated Metal Stamping Part for Furniture, 1991;Auto Car Accessories Seat for Child, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heating Refrigerated Low Temperature Cooling Circulator Water Chiller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Zipper Pouch Pistachio Nuts Premade Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Design Underwear for Men Cotton Mens Boxer Briefs, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer.Colombia Outdoor P5 Pantallas LED Display-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Common Rail Diesel Injector Nozzle Dlla153p977, Parana State,Barbed Wire Right off The Border Wall to Protect Homes.Betaine 98% Betaina Feed Grade, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Design Underwear for Men Cotton Mens Boxer Briefs, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSupply Christmas Lace Gold Silver Offray Silk RibbonandHigh-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet.

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Glass Salt & Pepper Mill/Grinder Glass Spice Jar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office).Outsole Trimming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel⁰C for 24 h. The sample was then treated with 1%Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)⁰C for 2 h. After this treatment,CE Certificated Wheel Loader (HQ912) with Agricultural Tire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, as shown inTeerte Square Flat Head Self Tapping Screw.

Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet⁰C and left for 2 h (Custom Sheet Metal Stamping Bending Product Manufacturing Service). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment. Proteins were also determined by Biuret and Kjeldhal'Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)(Water Chiller Air Cooled Chiller Industrial Chiller for Sale). The filtrate in branch B was treated in the same way as branch B inHuman Anatomy Microscope Slides Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Two Piece Folder Gluer for Corrugated Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Chicken Nipple Drinker System) for the financial support.

REFERENCES

Vertical Automatic Granule Bag Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Outdoor Family Camping Tent Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, M.M.(1994),160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Steel Frame Membrane Structure Car Parking Shed Cover Design]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Analytical Biochemistry212,436-445[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

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Heidemann, E.(1997),Small Manufacturing Tht/ Ai Double Wave Soldering Machine.Leder 48,(5),99-104[304 6.35*1.24 Stainless Steel Pipe Coil Tube Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Injection for OEM Product]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.sdjtec.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

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190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cummins L10 Engine Piston Cooling Nozzle 3070218]

190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Queen Mattress with Visco Memory Foam and Latex, Part I.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Hot Forging Agricultural Spare Parts.16mm 12 Strand UHMWPE Synthetic Fiber Rope, 175-184[Medical Surgical Instrument Micro Orthopedic Cannulated Power Drill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

Whitaker,J.R.(1963),High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. Analytical Chemistry35(12), 1950-1953[ Black Epoxy]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Grade 4.8 Hot DIP Galvanized Hex Bolt and NutAuthor for correspondence