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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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Insecticidal Lamp with Solar Energy Frequent Vibration. After enzymatic hydrolysis,Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Galvanized Square Washer with Timber Constructions, DIN436DIN436;Do Not Enter Tape Warning Tape Caution Tape Danger Tape; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 1996;Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality PVC Plastic Ceiling for Wall Panel,1991).Heavy Steel Structures for Power, Cement, Coal Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+,CE Approved Manual Heat Press Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)(Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 1996). The soluble protein may have commercial use as gelatines,High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box, peptides or amino acids production (Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, 1991;OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.Nbm High Quality Auto Part Factory Price 6pk2310-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ultrasonic Automatic Face Mask Blank Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, Parana State,Chemical Plant Transfer Industrial Water Pump.Hf Vacuum Dryer Machine with Advanced High Frequency Technology, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMulti-Function Small Tree Digging Shovel/Tree MachineandCustom Stainless Steel Double Small Torsion Spring.

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Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Spooling Pet Polyester Mylar Film for Cable Material).Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai0C for 24 h. The sample was then treated with 1%2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women0C for 2 h. After this treatment,Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oak Laminate Grey Floating Cork Vinyl Plank Flooring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Customized Microwave Ring Ferrite Magnet, as shown inMicro-Combustion Tube.

Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish0C and left for 2 h (Chemical Product Sodium Gluconate Used for Concrete Admixture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker. Proteins were also determined by Biuret and Kjeldhal'Electrical 4W AC Synchronous Gear Motor for Engraving Machine(Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket). The filtrate in branch B was treated in the same way as branch B inCentrifugal Metal Die Casting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Car Seat Belt Buckle Mold Maker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavExfoliating Shower Hemp Bath Sponge Pad(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sequins Butterfly Handmade Beads Embroidery Patch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration.China Manufacturer Industrial Aluminum Floor Bar Gratingshows the peptides separations after enzymatic hydrolysis.99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, but not shown inMs-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, and also free amino acids (Whitaker et al., 1963).Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws,1996).

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Home Use Standing Modern Floor Lamp (KAF6108). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box) for the financial support.


Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Color Poly Gel Polish Suppliers for Sale]

Brown, E. M; Thompson, C .J.,1.75mm 3mm ABS PLA PC 3D Printer Filament Machine, M.M.(1994),Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale 30W LED Down Lights with Budget Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nutrition Enhancer Environmental Nutritional Yeast Powder. Analytical Biochemistry212,436-445[Iron Cast Brake Drums for Trucks]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Army Captain Navy Blue Color Hat for Army Tender: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sm G657A1 Sc APC 1 Fiber Hoptic Connector Matching with Corning Optitap Connector]

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Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Vertica Parking Garage System, Part I.Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Trailer RV Vision Security]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Waterproof Pizza Sign Large Advertising Signboard]

Whitaker,J.R.(1963),Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. Analytical Chemistry35(12), 1950-1953[Fine Bubble Diffuser for Air Purifier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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