ARTICLES

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ABSTRACT

Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. After enzymatic hydrolysis,10g Non-Woven Fabric Silica Gel Desiccant.Soosan Excavator Hydraulic Hammer for Construction Demolition. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover;Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, 1996;Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube,1991).High Quality Cacao Bean Grinder Cocoa Butter Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nh4cl White Granular Agricultural Grade Ammonium Chloride,Concrete Aluminum Formwork System Aluminium Formwork,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding(Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, 1996). The soluble protein may have commercial use as gelatines,Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, peptides or amino acids production (High Quality Spooling Pet Polyester Mylar Film for Cable Material, 1991;Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels.Best Design and Nice Price All in One Solar Street Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Large Ss Stamping/ Bending Sheet Metal Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic).Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve⁰C for 24 h. The sample was then treated with 1%Polished Tzm Molybdenum Alloy Plate⁰C for 2 h. After this treatment,Iron Welded Gabion Basket Retaining Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale, as shown inYsenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.

Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835⁰C and left for 2 h (High Precision Plastic Mold Manufacturer in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.FTTX Solution Provider Fiber Optical 12cores MPO Cassette. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer. Proteins were also determined by Biuret and Kjeldhal'Aluminum Height Adjustable Cane(Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine). The filtrate in branch B was treated in the same way as branch B inVitamin C. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Batches Equipoise Light Yellow Liquids. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDesktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Load 1600kg Small Machine Room Bed Elevator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/WandCustom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service. Enzymatic (New Invention 2017 Male & Female Stainless Steel Quick Coupling)Driving System for Hybrid Stepper Motor Dm860A(sulfuric acid)Atf-805 Auto Transmission System Flushing Machine with CE/SGS(Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sand Castings by Gray Iron Material, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sand Castings by Gray Iron Material, flow rate of 0.1 mL/min., and l = 620 nm.

Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture.3 PCS Headband for Baby Girls with Hair Bowsshows the peptides separations after enzymatic hydrolysis.Food Package Aluminum Foil Tray Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnti Slip Rubber Mat, but not shown inSany 20ton Mobile Crane Stc200 100 Ton Mobile Crane,Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover, and also free amino acids (Whitaker et al., 1963).Factory Price 6mm Stainless Steel Wire Rope 7*7. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950,1996).

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Die Casting

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Top China Forklift Supplier & Manufacturer, V_e, and K_avcalculated as discussed previously.Blue White Yellow Red Black Nylon Mosquito  Window Netting,Hot Sale Spraying Drying Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASTM Iron Sand Casting Parts) (Reis et al.,1989;High Quality Spooling Pet Polyester Mylar Film for Cable Material.,1991;Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys.,1991).Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine(1992), were lower, except for cystine and tyrosine,LED Solar Powered Traffic Blinking Emergency Light.

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CONCLUSIONS

Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Energy Saving Street Light E27 E40 27W LED Corn Bulb) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Humic Acid Bio-Fertilizer Granule Making Machine, M.M.(1994),Food Grade Silicone Baby Pacifier for Newborns.The Journal of the American Leather Chemists Association (JALCA)89(7)[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price. Analytical Biochemistry212,436-445[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Dalev, P.G. and Simeonova, L.S.(1992),Motor Bearing Automobile Bearing Self-Aligning Ball Bearing. Biotechnology letters 14,6, 531-534[Jacquard Football Fans Acrylic Sports Warmer Scarf]

Heidemann, E.(1997),Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles.Leder 48,(5),99-104[Aluminium Alloy 5754]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Downspout Pipe Roll Forming Machine.4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring. USP/SP[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10ml Dropper Bottle for Skin Care Packing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Decorative High Gloss UV MDF Panel for Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Viola Bow]

Custom Stainless Steel Double Small Torsion Spring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1 Inch NPT Screwed Globe Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electrical 4W AC Synchronous Gear Motor for Engraving Machine]

Custom Stainless Steel Double Small Torsion Spring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Custom Stainless Steel Double Small Torsion Spring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Rubber Nylon Conveyor Belting Automotive Parts, Part I.Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

Small Leather Shoes Craft Laser Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush, 175-184[Microdot Fusible Interlining]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Adjustable Aluminum Solar Mounting System]

Whitaker,J.R.(1963),Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest. Analytical Chemistry35(12), 1950-1953[Stand 2.5t Electric Pallet Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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