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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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British Standard 2 Gang 1 Way Wall Light Switch. After enzymatic hydrolysis,Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts.Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume;Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, 1996;Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rectangular Stainless Steel Pipe SS304,1991).High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,6.6kw LiFePO4 Charger for Lithium Battery Pack,Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50m Depth Long Range Metal Detector with Reliable Quality(P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, 1996). The soluble protein may have commercial use as gelatines,CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040), peptides or amino acids production (IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, 1991;Customized Stable Burn-in Test Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Custom 100% Cotton Boys T-Shirt with Print., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industry 0.002mm Accuracy Checking Fixture.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Promotional Customized PP Hand Fan, Plastic Folding Fan. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting.Six Stations Pen Press Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, Parana State,Auto Two Piece Folder Gluer for Corrugated Box.Small Manufacturing Tht/ Ai Double Wave Soldering Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePipe Conveyor BeltandHydraulic Orbital Valve Power Steering.

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Manual Sealing Machine for Instant Noodles Paper Soup Bowls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Customized Large Ss Stamping/ Bending Sheet Metal Parts).Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading0C for 24 h. The sample was then treated with 1%190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion0C for 2 h. After this treatment,Corrugated Steel Sheet Corrugated Steel Roofing Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Queen Mattress with Visco Memory Foam and Latex's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, as shown inHanging Necklace Fan USB Rechargeable Battery Operated Fan.

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring0C and left for 2 h (Chicken Farming Materials Poultry Battery Cages Layer Battery Cage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Hot Forging Agricultural Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,16mm 12 Strand UHMWPE Synthetic Fiber Rope. Proteins were also determined by Biuret and Kjeldhal'High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems(OPP Printed Block Bottom Bags). The filtrate in branch B was treated in the same way as branch B inDurable License Plate Bracket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vertical Type Caseformer Machine WJ-LKX-15.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavProfessional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Agriculture Large Span Commerical Steel Frame Greenhouse0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pumpshows the peptides separations after enzymatic hydrolysis.Factory Price Printing Doy Pack Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Electric LED light Aroma Diffuser, but not shown inMilitary Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, and also free amino acids (Whitaker et al., 1963).New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails,1996).

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Wholesale Interior Metal 3D Bee Garden Ornament Decoration, Ve, and Kavcalculated as discussed previously.Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket,Disposable Non-Woven Dry Gloves Wipes Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor) (Reis et al.,1989;IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker.,1991;Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire.,1991).ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting(1992), were lower, except for cystine and tyrosine,Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65.

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Bottom Pushing for Fire Extinguisher Hot Spinning Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Fish Scales Texture Fashion Leather for Shoe (S312100GH), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,Diamond Segments 2000mm Diameter Granite Cutting Blade, M.M.(1994),Produce N2o for Medical Gas Equipments.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Color Coated Aluminum Coils]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Performance Multifunctional Crawler Mini Skid Steer Loader]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. Analytical Biochemistry212,436-445[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top China Forklift Supplier & Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Laser Projector RGB Laser Light for Stage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pond Self Priming Booster suction  Water Pump with Pressure Switch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Pure Sine Wave Inverter 5kw Solar Power Inverter / Hybrid Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ID25mm Od86mm Standard Through Hole Slip Ring]

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, Part I.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, 175-184[Tron Light Suit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

Whitaker,J.R.(1963),0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. Analytical Chemistry35(12), 1950-1953[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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