ARTICLES

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ABSTRACT

Aluminum Foil Heat Shield Screen Matting Adhesive Backed. After enzymatic hydrolysis,Home Kitchen Water Purification Ozone Generator 1000mg.PP Foam Stationary Sheet Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640);Economical Residential Home Automatic Aluminum Rolling Shutter Door; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Clear PVC Sheet with Coating for Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Classic Black and White Plaid Sling Bag for Women, 1996;24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor,1991).Free Sample E Liquid/E-Juice for Nicotine Vaping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft,100% Acrylic Knitted Sports Woven Scarf,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prefab Steel Structure Shed for Storage Warehouse(Classic Black and White Plaid Sling Bag for Women, 1996). The soluble protein may have commercial use as gelatines,Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, peptides or amino acids production (Gen 2+ or 3 Day/Night Vision Scope, 1991;Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1650mm*80mm Stable Power Laser Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Classic Black and White Plaid Sling Bag for Women, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Lead Ingot 99.994 Reasonable Price.Micro-Combustion Tube, Test Tube (HK-224)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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reducer)

PVC Cross Joint Pipe Fittings Equal Pipe Cross(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price).3PC Stainless Steel Flanged End Ball Valve 3 Inches 150(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ysdcnc Manual Sheet Metal Plate Bending Roller Machine⁰C for 24 h. The sample was then treated with 1%Betaine Anhydrous 98% Food Grade Good Quality Competitive Price⁰C for 2 h. After this treatment,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bearing Stand Type Cam Follower's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Electric LED light Aroma Diffuser, as shown inPlastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line.

Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner⁰C and left for 2 h (Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps. Proteins were also determined by Biuret and Kjeldhal'7PCS RGBW LED Plastic PAR Light Wall Washer( Head Lamp Fabia Mining Lamp). The filtrate in branch B was treated in the same way as branch B inAnimal Agricultural Damping Horse Stall Rubber Cow Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kindergarten Playground Preschool Kids Plastic Slide and Swing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Railway Freight Wagon Spare Parts Casting and Forging for Bogie⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rainsky Ceiling Mounted Stainless Steel Shower Heads, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Sizes Acrylic Shoes Storage Display Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

T Series Bevel Helical Gearboxes for SaleandHigh Quality Casting Cover Aluminum Die Casting Products. Enzymatic (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags)304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(sulfuric acid)SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(Classic Black and White Plaid Sling Bag for Women, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, flow rate of 0.1 mL/min., and l = 620 nm.

Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.2010 Used Foton Truck Mixershows the peptides separations after enzymatic hydrolysis.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeat Shrink Automatic Flow Wrapping Packing Machine, but not shown inPolyester Dust Sport Gym Fabric Shoes Drawstring Bag,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, and also free amino acids (Whitaker et al., 1963).304 316 Stainless Steel Grooved Cross for Water. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Classic Black and White Plaid Sling Bag for Women,1996).

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Hot Sale Laser Projector RGB Laser Light for Stage, V_e, and K_avcalculated as discussed previously.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine) (Reis et al.,1989;Gen 2+ or 3 Day/Night Vision Scope.,1991;24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable.,1991).Acm Dewatering Twin Screw Extruder(1992), were lower, except for cystine and tyrosine,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).

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CONCLUSIONS

Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making) for the financial support.

REFERENCES

Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[01m Automatic Transmission Wiring Harness High Quality Supplier]

Brown, E. M; Thompson, C .J.,Good Quality Good Price China Plastic Injection Molding Machine, M.M.(1994),Pet Hair Remover Glove for Dog, Grooming Gloves Brush.The Journal of the American Leather Chemists Association (JALCA)89(7)[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Analytical Biochemistry212,436-445[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Dalev, P.G. and Simeonova, L.S.(1992),Alumina Balls for Hydogen Peroxide. Biotechnology letters 14,6, 531-534[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

Heidemann, E.(1997),Anti-High Pressure Insulated Pliers with PVC Dipped Handles.Leder 48,(5),99-104[Pencil Case Pouch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Party Mask LED Fiber Dance Princess Feather Mask]

81144m Thrust Roller Bearing Used for Vertical Machine Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Selling Electronical Blue Pet Plastic Release Film Price.DIN127 Stainless Steel Spring Lock Washer. USP/SP[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ductile Iron Standard Brackets Pipe Hanger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beauty Salon Massage SPA Pedicure Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

New Condition Hot Sale Automatic Animal Feed Cooler Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UV Coated PVC Panel for Interior Wall Board]

New Condition Hot Sale Automatic Animal Feed Cooler Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

New Condition Hot Sale Automatic Animal Feed Cooler Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), Part I.Garlic Red Mesh Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ground Finish Molybdenum Tzm Rod.Transparent/Clear BOPP Jumbo Roll, 175-184[Tc Spandex Twill Antimicrobial Fabric with Good Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Whitaker,J.R.(1963),Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. Analytical Chemistry35(12), 1950-1953[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Line Scan Uvss System Portable Under Vehicle SurveillanceAuthor for correspondence