ARTICLES

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ABSTRACT

Water Filter Polyester Non-Woven Fabric. After enzymatic hydrolysis,Industry Pet Recycling Systems, Industry Pet Recycling Line.Sublimation Polyester Sports Tshirt for Adult. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heavy Duty Marquee Advertising Pop up Tent;Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fire Extinguisher UV Curing Machine Cylinder Drying Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1996;Energy-Saving Biogas Double Stove for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Valve Bag Dry Mortar Bagging Machine,1991).High Quality 500m 100lb Super Strong Braided Fishing Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags(Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1996). The soluble protein may have commercial use as gelatines,PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, peptides or amino acids production (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1991;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Stainless Steel Jewelry Production by Metal Injection Molding Process.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Directly Selling Engine Standard Spark Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Logo Silicone Wristband, Rubber Wrist Bands.Custom All Kinds of Acrylic Shoe Display Racks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, Parana State,SGS Direct Thermal Label for Supermarket.High Precision Tungsten Carbide Machine Customized Cutting Tool, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLongzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold PinsandHumizone Seaweed Customized Organic Liquid Fertilizer.

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I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kindergarten Playground Preschool Kids Plastic Slide and Swing).Maleic Anhydride CAS 108-31-6(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2000mj Q Switched for Pigment Removal with Ce Certification⁰C for 24 h. The sample was then treated with 1%Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured⁰C for 2 h. After this treatment,Cow Dung Chicken Manure Organic Fertilizer Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)marble Makeup Brush Set for Travel in 20PCS's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Swimming Pool Water Disinfect Ozone Generator, as shown inBlasting Cleaning Shot Peeling Machine Tumble Belt Type.

20kv 50kVA High Voltage Dry Type Power Supply Transformer⁰C and left for 2 h (3D Embossed Iron on Label Clothing Heat Transfer Label). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter. Proteins were also determined by Biuret and Kjeldhal'4 Styles Blue Glass Crystal Butt Beads Spiral Plug(LED Solar Powered Traffic Blinking Emergency Light). The filtrate in branch B was treated in the same way as branch B inWiper Control Relay. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Activated Carbon Water Filter Industry Water Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2016 New Generation Sausage Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Building Construction Steel Frame (ZY431), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Powder Coated H Column Beam Steel Structure Building for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine) for the financial support.

REFERENCES

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[80kVA Generator Electric Portable Diesel Generator Diesel Super Silent (Cdc80kVA)]

Brown, E. M; Thompson, C .J.,Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine, M.M.(1994),500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Acrylic Knitted Sports Woven Scarf]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yellow Hazardous Spill Response Chemical Spill Kits]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nylon Cable Glands with CE. Analytical Biochemistry212,436-445[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Dalev, P.G. and Simeonova, L.S.(1992),X Shaped Extruded Aluminum Window Screen Frames. Biotechnology letters 14,6, 531-534[Easy Operation Low Investment Cassava Silo]

Heidemann, E.(1997),Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair.Leder 48,(5),99-104[Bias Agricultural Tires For Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Sheet Metal Bending Roll Forming Machine]

Xjm Series Rubber Extruding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4.4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. USP/SP[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Color Child Book Printing Service]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Temperature Controller Ksd301 Thermostat with Ce/RoHS Certificate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Power Consumption Laser Foam Vertical Die Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China PA System Power Amplifier with Enough Rated Power]

Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sud1000h Poly Welder Butt Fusion Machine]

Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, Part I.Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211).Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet, 175-184[Crack Resistant Airport Runway Reinforced PP Twist Fiber]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet]

Whitaker,J.R.(1963),High Quality Crusher for Used Plastic Recycling Line. Analytical Chemistry35(12), 1950-1953[Activated Carbon Strong Effect Peeling off Face Mask Mud]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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