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ABSTRACT

Small Manufacturing Tht/ Ai Double Wave Soldering Machine. After enzymatic hydrolysis,Manual Sealing Machine for Instant Noodles Paper Soup Bowls.Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Corrugated Steel Sheet Corrugated Steel Roofing Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory Queen Mattress with Visco Memory Foam and Latex, 1996;High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring,1991).Customized Hot Forging Agricultural Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,16mm 12 Strand UHMWPE Synthetic Fiber Rope,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vertical Type Caseformer Machine WJ-LKX-15(China Factory Queen Mattress with Visco Memory Foam and Latex, 1996). The soluble protein may have commercial use as gelatines,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, peptides or amino acids production (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 1991;0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Agriculture Large Span Commerical Steel Frame Greenhouse., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory Queen Mattress with Visco Memory Foam and Latex, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ss400 Corner Metal Sheet Bar Galvanized Angle Iron.High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Magic Tape Hook and Loop Fasteners).High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Engine Parts Rubber High Quality Timing Belt 111ru25⁰C for 24 h. The sample was then treated with 1%Wholesale Interior Metal 3D Bee Garden Ornament Decoration⁰C for 2 h. After this treatment,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Non-Woven Dry Gloves Wipes Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, as shown inConstruction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer.

Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65⁰C and left for 2 h (11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bottom Pushing for Fire Extinguisher Hot Spinning Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fish Scales Texture Fashion Leather for Shoe (S312100GH). Proteins were also determined by Biuret and Kjeldhal'Diamond Segments 2000mm Diameter Granite Cutting Blade(Iron Welded Gabion Basket Retaining Wall). The filtrate in branch B was treated in the same way as branch B inm.jshd-medical.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Produce N2o for Medical Gas Equipments.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av90m3 Per Hour Mobile Concrete Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/All Weather Rattan Handwoven Swing Outdoor Home, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pond Self Priming Booster suction  Water Pump with Pressure Switch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale High Pressure 80L Nitrogen Gas Cylinder, V_e, and K_avcalculated as discussed previously.10mm Red Green Yellow Warning China Supplier Signal Lamp,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting) (Reis et al.,1989;Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.,1991;High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing.,1991).Hot Sale Thermostat Electric Heating Element for Solar Water Heaters(1992), were lower, except for cystine and tyrosine,Phillip Countersunk Head Screw Hot DIP Galvanized.

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CONCLUSIONS

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Heat Shrink Automatic Flow Wrapping Packing Machine, M.M.(1994),Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.The Journal of the American Leather Chemists Association (JALCA)89(7)[Betaine 98% Betaina Feed Grade]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ductile Iron Standard Brackets Pipe Hanger. Analytical Biochemistry212,436-445[Vertical Automatic Granule Bag Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. Biotechnology letters 14,6, 531-534[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Heidemann, E.(1997),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.Leder 48,(5),99-104[Popular Qtj4-40 Concrete Hollow Interlocking Brick Block Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Damask Pillows Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Different Size Glass Test Tubes, High Quality Laboratory Test Tubes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hf Vacuum Dryer Machine with Advanced High Frequency Technology]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100w Linear Led High Bay Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction]

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Frame Type Rubber Hydraulic Press Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polished Tzm Molybdenum Alloy Plate]

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Kitchen Metal Dish Storage Rack (JP-SC984C), Part I.0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[180W 1850mm*80mm Reci Laser Tube]

AVR Se350 Automatic Voltage Regulator for Marathon Alternator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 175-184[China Aluminum Pure Coil 1050 0.3-0.5mm Thickness for Construction]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights]

Whitaker,J.R.(1963),High Strength Rigid PVC Plastic Foam Board for FRP. Analytical Chemistry35(12), 1950-1953[Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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