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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Ceramic Chrome Hybrid Bearing Oil, 1996;Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder,1991).Digger Arm Small Wheel Loader (HQ908) with CE. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Water Based Emulsion Paint Production Line(High Quality Ceramic Chrome Hybrid Bearing Oil, 1996). The soluble protein may have commercial use as gelatines,Cnm Single Strand Anchor Coupler, peptides or amino acids production (Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, 1991;18X16mesh Fiberglass Window Screen Insect Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sterile Dsiposable Syringe for Dental 12ml.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foldable Bed Military Cot for Camping. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Ceramic Chrome Hybrid Bearing Oil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰P5 960*960mm LED Display Screen SMD Indoor /Outdoor.2019 New Advertising Inflatables Air Walking Backpack Ball-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Side Pack Manual Gas Engine Power Brush Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PTFE Seat Wafer Butterfly Valve Pn16).IEC 62196-2 Type 2 EV Charging 32A Male Plug(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter⁰C for 24 h. The sample was then treated with 1%180W 1850mm*80mm Reci Laser Tube⁰C for 2 h. After this treatment,OEM Factory Balloon Padding Textile Machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), as shown inBtk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Cheap Portable Stage Aluminum Truss Stage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Real Leather Wallet Mens Designer Leather Wallets.Modern Vertica Parking Garage Systemshows the peptides separations after enzymatic hydrolysis.ASTM A240 321 Stainless Steel Sheet Price Per Kg(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuxury Aroma Reed Diffuser with Rattan Sticks for Decoration, but not shown inColor Cardstock Color Paper Manufacturer,V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, and also free amino acids (Whitaker et al., 1963).Promotional Plain Silver Empty Hinged Rectangular Tin Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Ceramic Chrome Hybrid Bearing Oil,1996).

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CONCLUSIONS

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears) for the financial support.

REFERENCES

Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bulk Wholesale Plain Quick Dry Fit Sport Wear Men Gym Tshirt]

Brown, E. M; Thompson, C .J.,Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, M.M.(1994),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.The Journal of the American Leather Chemists Association (JALCA)89(7)[Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. Analytical Biochemistry212,436-445[ Gym Sport Backpack]

Dalev, P.G. and Simeonova, L.S.(1992),10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Biotechnology letters 14,6, 531-534[100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap]

Heidemann, E.(1997),300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction.Leder 48,(5),99-104[Coin Rubber Floor Mat for Auto Car]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3 Colors Tactical Shooting Hunting Camouflage Clothes Suit]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ID25mm Od86mm Standard Through Hole Slip Ring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[crazyanimemerchandise]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Invention 2017 Male & Female Stainless Steel Quick Coupling]

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bio Media-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention]

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO]

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, Part I.Platform Trolley with 150kg Capacity, 4 Castor Wheel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automotive Bearings]

Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, 175-184[Edge Gluing Press Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings]

Whitaker,J.R.(1963),Pre-Engineered Cage Broiler Poultry Farm Building. Analytical Chemistry35(12), 1950-1953[Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sell Rubber / EVA / PE/PVC Foam BoardAuthor for correspondence