ARTICLES

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ABSTRACT

Pneumatic Air Driven Liquid Booster Pump. After enzymatic hydrolysis,Heavy Duty Wood Door Calibrating Wide Belt Sander.Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK;X-Humate 95% Min Super Sodium Humate (Nut Moradant); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Women Summer Swimsuit Chest Zip Bathing Suit, 1996;Hemp Seed Oil Body Lotion Moisturizes The Skin., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh,1991).Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UTP CAT6 48 Ports Vertical Termination Patch Panel,Prefab Expandable Luxury Living Container House Prefabricated House,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box(Women Summer Swimsuit Chest Zip Bathing Suit, 1996). The soluble protein may have commercial use as gelatines,Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, peptides or amino acids production (Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1991;Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Hexagonal Wire Mesh Gabion / Gabion Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Must 4kw 2HP Pump Power Inverter Ags Bts Available. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Women Summer Swimsuit Chest Zip Bathing Suit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Small Steel Silo for Poultry Feed.Good Price Nasal Paste Packaging Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder).Ms Gi Pipe Dn15~Dn400 Gi Pipe List(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low-E Insulating Glass Curtain Wall⁰C for 24 h. The sample was then treated with 1%Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024⁰C for 2 h. After this treatment,Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Design Underwear for Men Cotton Mens Boxer Briefs's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nylon Balance Bike with Water Transfer Printing Malaysia, as shown inWomens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+.

Anti Slip Rubber Mat⁰C and left for 2 h (Hot Sale Thermostat Electric Heating Element for Solar Water Heaters). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor. Proteins were also determined by Biuret and Kjeldhal'Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair(Manufacturer Custom Precision Aluminum Die Casting LED Light Housing). The filtrate in branch B was treated in the same way as branch B inChina Aramid Flame Retardant Viscose Blended Polo Shirt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomotive Perforated Metal Stamping Part for Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Zipper Pouch Pistachio Nuts Premade Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Chicken Nipple Drinker System.Degradable PLA Plastic Bottleshows the peptides separations after enzymatic hydrolysis.Industrial Hydraulic Ice Grape Press Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, but not shown inNylon Cable Glands with CE,Common Rail Diesel Injector Nozzle Dlla153p977, and also free amino acids (Whitaker et al., 1963).Betaine 98% Betaina Feed Grade. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Women Summer Swimsuit Chest Zip Bathing Suit,1996).

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CONCLUSIONS

Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate) for the financial support.

REFERENCES

ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Extrusion Nutritional Artificial Rice Machine]

Brown, E. M; Thompson, C .J.,Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), M.M.(1994),Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Ultra Slim Elevator Digital Signage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw. Analytical Biochemistry212,436-445[Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Two Piece Folder Gluer for Corrugated Box. Biotechnology letters 14,6, 531-534[CNC Fiber Laser Cutting Machine for Sheet and Tube]

Heidemann, E.(1997),British Standard 2 Gang 1 Way Wall Light Switch.Leder 48,(5),99-104[FC Adapter Type Fixed Fiber Optic Attenuator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small Manufacturing Tht/ Ai Double Wave Soldering Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chenille Microfiber Mop Head]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ADSS Newest Ultra Therapy Skin Tightening 3D Hifu]

Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Common Rail Injector Tester]

Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, Part I.Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Rectangular Stainless Steel Pipe SS304, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry.6.6kw LiFePO4 Charger for Lithium Battery Pack, 175-184[Disposable Espresso Shot Cups]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Whitaker,J.R.(1963),Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. Analytical Chemistry35(12), 1950-1953[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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