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ABSTRACT

Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. After enzymatic hydrolysis,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, 1996;Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Ceramic Chrome Hybrid Bearing Oil,1991).Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder,Digger Arm Small Wheel Loader (HQ908) with CE,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer(Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, 1996). The soluble protein may have commercial use as gelatines,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, peptides or amino acids production (Water Based Emulsion Paint Production Line, 1991;Cnm Single Strand Anchor Coupler, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.18X16mesh Fiberglass Window Screen Insect Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sterile Dsiposable Syringe for Dental 12ml.Foldable Bed Military Cot for Camping-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:P5 960*960mm LED Display Screen SMD Indoor /Outdoor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy⁰C and left for 2 h (24k Gold Whitening Anti-Spot Mask). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. Proteins were also determined by Biuret and Kjeldhal'Outdoor Concert Stage Equipment Aluminum Stage Portable Stage(Low-E Insulating Glass Curtain Wall). The filtrate in branch B was treated in the same way as branch B inCompetitive Price PVC Resin Sg5/K67 Manufacturer in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%22*30mm 12SMD 5050 LED Reading Light for Car. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Cheap Portable Stage Aluminum Truss Stage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/One Arm Motorized Multifunction Hospital Equipment for China Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Punching Bending Welding Steel Work Fabrication) for the financial support.

REFERENCES

CNC Fiber Laser Cutting Machine for Sheet and Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bamboo Pants]

Brown, E. M; Thompson, C .J.,Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, M.M.(1994),Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.The Journal of the American Leather Chemists Association (JALCA)89(7)[50kg Unmanned Two-Way Magnetic Tape Guided Agv]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Boned Polyester Woven Faux Suede Sofa Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. Analytical Biochemistry212,436-445[High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free]

Dalev, P.G. and Simeonova, L.S.(1992),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Biotechnology letters 14,6, 531-534[Wholesale China Merchandise 12V Horizontal Mini Camping Fridge]

Heidemann, E.(1997),High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.Leder 48,(5),99-104[Cake Baking Pans]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Wine Carrier Bag Gift Box Tote]

Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. USP/SP[Our Perforated Aluminum & Solid Panel for New Office Wall Cladding]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Foldable Kids Walking Chair Toys Baby Walker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-BS 5163 Ductile Iron Rising Stem Gate Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Light Relay For Light Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Manufacturer Customized Microwave Ring Ferrite Magnet]

CNC Aluminum Sheet Press Brake, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ID25mm Od86mm Standard Through Hole Slip Ring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal]

CNC Aluminum Sheet Press Brake,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending]

CNC Aluminum Sheet Press Brake, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, Part I.Bio Media. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign]

Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Platform Trolley with 150kg Capacity, 4 Castor Wheel.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 175-184[Black Clothes Dye]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria]

Whitaker,J.R.(1963),LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Analytical Chemistry35(12), 1950-1953[Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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