ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Water Treatment Anthracite. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Heat Treated Welding Protection Glassfiber Cloth Ht800

ABSTRACT

Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. After enzymatic hydrolysis,Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.Heat Pump Non-Programmable HVAC Room Thermostat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, 1996;Injector Pump Calibration Test Bench., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASME/ASTM Butt-Welding Stainless Steel Plate Flange,1991).500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings,Polyester / Polypropylene Woven Geotextile for Road Construction,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Granite Stone Jaw Crusher of Mining Machine(Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, 1996). The soluble protein may have commercial use as gelatines,Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, peptides or amino acids production (Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, 1991;The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale All Kinds Cheap Plush Animal Baby Slippers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane.Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China PA System Power Amplifier with Enough Rated Power(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Superior Watertightness JS Polymer Cement Waterproof Coating for Basement).Export Grade Citric Acid Monohydrate as Detergent(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace⁰C for 24 h. The sample was then treated with 1%Familu Use Mini Small Pig Feed Pellet Mill⁰C for 2 h. After this treatment,3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, as shown inNew Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel.

Hairdressing Equipment Takara Belmont Barber Chair High Quality⁰C and left for 2 h (Silicone Cake Mold Sinle Cupcake DIY). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Buy Single Item Moving Bee Eye Light Lowly Price. Proteins were also determined by Biuret and Kjeldhal'High Quality Customized Aluminum Foil Shisha Sheet(Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant). The filtrate in branch B was treated in the same way as branch B inCad Jewelry Design Ring Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRectangular Box Facial Tissue Paper Fk-110(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Home and Office PVC Vinyl Flooring Conductive Plank FlooringandQ32 Series Tumble Belt/Crawler Shot Blasting Machine. Enzymatic (Galvanized Bar Grating)High Quality Custom Self Adhesive Leather Patch for Jackets(sulfuric acid)Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale(Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%380mm Working Area Concrete Diamond Floor Edge Grinder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%380mm Working Area Concrete Diamond Floor Edge Grinder, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Aluminum Low Profile Adjustable Bracket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Airtight Candy Bin with Scoop) for the financial support.

REFERENCES

Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heavy Duty 65mn Steel 304 304L Stainless Steel Crimped Wire Mesh for Mine Sieve]

Brown, E. M; Thompson, C .J.,High Quality Acrylic Beer Display Racks with LED, M.M.(1994),Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C).The Journal of the American Leather Chemists Association (JALCA)89(7)[Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Baby Feeding Chair Plastic Chair for Feeding Hebei China. Analytical Biochemistry212,436-445[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

Dalev, P.G. and Simeonova, L.S.(1992),Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle. Biotechnology letters 14,6, 531-534[Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier]

Heidemann, E.(1997),Plastic Injection Molding for Auto Parts.Leder 48,(5),99-104[Roma White Granite Slabs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Round Type Twin Screw Extruder Barrel Manufacturer.ASA-UPVC Anti-Corrosive Composite Roof Tile. USP/SP[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Grey Full-Zip Cotton Mens Hoodies]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pressure bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Sexy Mesh Transparent Female Running Tank Top with Hood Custom, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Color Cardstock Color Paper Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24k Gold Whitening Anti-Spot Mask]

Sexy Mesh Transparent Female Running Tank Top with Hood Custom,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low-E Insulating Glass Curtain Wall]

Sexy Mesh Transparent Female Running Tank Top with Hood Custom, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, Part I.Home and Office PVC Vinyl Flooring Conductive Plank Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Precision Engineering Parts Bearing Support Unit for Ball Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection.165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, 175-184[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Enameled Mini Cast Iron Casserole]

Whitaker,J.R.(1963),Silicone Cake Mold Sinle Cupcake DIY. Analytical Chemistry35(12), 1950-1953[Modular Portable Shipping Container Lkit Building Home Villa House]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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