ARTICLES

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ABSTRACT

Heavy Steel Structures for Power, Cement, Coal Plant. After enzymatic hydrolysis,Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+.CE Approved Manual Heat Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, 1996;160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless,1991).AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner,Nbm High Quality Auto Part Factory Price 6pk2310,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ultrasonic Automatic Face Mask Blank Making Machine(OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, 1996). The soluble protein may have commercial use as gelatines,Manufacturer of Soda Ash Dense 99 2% Price, peptides or amino acids production (Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525, 1991;Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chicken Slaughtering Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hf Vacuum Dryer Machine with Advanced High Frequency Technology. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving).Electrical 4W AC Synchronous Gear Motor for Engraving Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Car Seat Belt Buckle Mold Maker⁰C for 24 h. The sample was then treated with 1%DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water⁰C for 2 h. After this treatment,Exfoliating Shower Hemp Bath Sponge Pad. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), as shown inCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

Sequins Butterfly Handmade Beads Embroidery Patch⁰C and left for 2 h (Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. Proteins were also determined by Biuret and Kjeldhal'Adjustable Aluminum Solar Mounting System(Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine). The filtrate in branch B was treated in the same way as branch B inPatterned Vinyl Floor Tiles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Wireless Cheapest Android 2D Barcode Scanner.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Price Diamond Wire Mesh Chain Link Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Heating Mantle 5L(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides. Wire Diameter 18AWGshows the peptides separations after enzymatic hydrolysis.Sleeved Wire Harness for Industry Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3 Layer Round Tea Tin with Air Tight Lid, but not shown inHot Selling Furring Channel Fittings Suspended Ceiling Accessories,Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine, and also free amino acids (Whitaker et al., 1963).Home Use Standing Modern Floor Lamp (KAF6108). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner,1996).

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Nutrition Enhancer Environmental Nutritional Yeast Powder, V_e, and K_avcalculated as discussed previously.Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring,CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse) (Reis et al.,1989;Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525.,1991;160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain.,1991).New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board(1992), were lower, except for cystine and tyrosine,Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase.

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CONCLUSIONS

High Quality Army Captain Navy Blue Color Hat for Army Tender. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Oak Laminate Grey Floating Cork Vinyl Plank Flooring) for the financial support.

REFERENCES

Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Factory Cross Type Concertina Razor Barbed Tape Wire]

Brown, E. M; Thompson, C .J.,High Efficiency Mini-Pleat HEPA Filter (H13/H14), M.M.(1994),Modern Vertica Parking Garage System.The Journal of the American Leather Chemists Association (JALCA)89(7)[2000mj Q Switched for Pigment Removal with Ce Certification]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test. Analytical Biochemistry212,436-445[Industrial Activated Carbon Water Filter Industry Water Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),Galfan Wire (Aluminium-Zinc Alloy coated). Biotechnology letters 14,6, 531-534[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof EVA Rubber Roofing Material for Shingle Building]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Side Aluminum Side Printed Circuit Board PCB Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Automatic Three Side Sealing Facial Mask Bag Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50kg Unmanned Two-Way Magnetic Tape Guided Agv-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Counter Top Working Table Chiller]

Automatic Three Side Sealing Facial Mask Bag Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Diesel Engine Type Zzs Sand Vibrating Screen]

Automatic Three Side Sealing Facial Mask Bag Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Manufacture Commercial Use Spice Grinder, Part I.Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Condoms/Sex Toys/Capsule Toy Vending Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304 316 Stainless Steel Grooved Cross for Water]

Whitaker,J.R.(1963),Professional Stainless Steel Ethanol Processing Plant From Cassava. Analytical Chemistry35(12), 1950-1953[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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