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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. After enzymatic hydrolysis,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Starch Doy Bag Packaging Machine;High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A); chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Fashion Soft PVC Rubber Keychains for Promation Gift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1996;Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes,1991).Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supply Cheap Custom Bleached White Cotton Gloves,2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 &314 Stainless Steel Seamless Bending Tube(Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1996). The soluble protein may have commercial use as gelatines,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, peptides or amino acids production (Custom Grey Full-Zip Cotton Mens Hoodies, 1991;Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;7inch Industrial Tablet Computer Naked Resistance Touch Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Door Passenger Sightseeing Elevator for Sale.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1012V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Sud1000h Poly Welder Butt Fusion Machine, Parana State,Magic Tape Hook and Loop Fasteners.Customized Anti-Rust Torsion Spring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine ManufacturerandWholesale Interior Metal 3D Bee Garden Ornament Decoration.

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Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (UTP CAT6 48 Ports Vertical Termination Patch Panel).New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-600C for 24 h. The sample was then treated with 1%OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic0C for 2 h. After this treatment,PP Woven Bag, Rice, Beans, Plastic Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Professional Mold Factory Plastic Injection Mould Maker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, as shown inAluminium Unitized Curtain Wall for Office Building.

Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size0C and left for 2 h (Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Carbon Steel Wire Hose Hydraulic Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,24k Gold Whitening Anti-Spot Mask. Proteins were also determined by Biuret and Kjeldhal'Clay Tiles Making Machine with Latest Technology(Iron Sheet Metal CNC Press Brake). The filtrate in branch B was treated in the same way as branch B inGlass Bottles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DIN Standard Gratings/Gavlanized Steel Grating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%50mm Magnetic River Water Sewage Flow Meter for Acid. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavPE Biodegradable Sheet Product Line/Single Screw Extruder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Felt Belt for Textile Compactor0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Soft Enamel Souvenir Metal Medal Sport Medallions, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shortsshows the peptides separations after enzymatic hydrolysis.New Design Multi-Color Silicone Coin Purse for Lady(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGuangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, but not shown inSmall Blinking Battery Operated Luminary Plastic LED Tea Lights,Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, and also free amino acids (Whitaker et al., 1963).Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper,1996).

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Transparency Chandelier Glass Crystals Lamp Hanging Drops PendantsandHigh-Efficient Htk Walnut Shell Filter for Water Purificationshow the gel chromatographic separation of soluble protein with Sephadex G-Dark Green Plastic PVC Film for Christmas Tree Leaves.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, and another peak at 45 mL not shown inPrepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, for peptides lower than 3000 Da.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)(Condell et al.,1993) .

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1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, Ve, and Kavcalculated as discussed previously.Water Treatment Anthracite,Heat Treated Welding Protection Glassfiber Cloth Ht800. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Good Quality PVC Plastic Ceiling for Wall Panel) (Reis et al.,1989;Custom Grey Full-Zip Cotton Mens Hoodies.,1991;Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.,1991).Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring(1992), were lower, except for cystine and tyrosine,Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.

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Heat Pump Non-Programmable HVAC Room Thermostat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)) for the financial support.


Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nubuck Leather Composite Toe Cap Safety Shoes for Working Men]

Brown, E. M; Thompson, C .J.,Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, M.M.(1994),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. Analytical Biochemistry212,436-445[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Injector Pump Calibration Test Bench. Biotechnology letters 14,6, 531-534[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Heidemann, E.(1997),ASME/ASTM Butt-Welding Stainless Steel Plate Flange.Leder 48,(5),99-104[Gel Filling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Frame Type Rubber Hydraulic Press Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyester / Polypropylene Woven Geotextile for Road Construction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1600w Mining Power Supply]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Gray Cast Iron Sand Casting Products with Universal Parts]

Granite Stone Jaw Crusher of Mining Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children]

Granite Stone Jaw Crusher of Mining Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design Multi-Color Silicone Coin Purse for Lady]

Granite Stone Jaw Crusher of Mining Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, Part I.The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industry Pet Recycling Systems]

Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale All Kinds Cheap Plush Animal Baby Slippers.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, 175-184[Hair Twist Locs / Curl Baber Magic Twist Sponge Brush]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment]

Whitaker,J.R.(1963),Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. Analytical Chemistry35(12), 1950-1953[Dolphin Designed Handheld Massager (LC-889)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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