High Speed Automatic Packing Machine (GK-650 BA)

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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Disposable Non-Woven Dry Gloves Wipes Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting


Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. After enzymatic hydrolysis,Bottom Pushing for Fire Extinguisher Hot Spinning Machine.Fish Scales Texture Fashion Leather for Shoe (S312100GH). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Diamond Segments 2000mm Diameter Granite Cutting Blade;Produce N2o for Medical Gas Equipments; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (90m3 Per Hour Mobile Concrete Pump, 1996;China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node,1991).All Weather Rattan Handwoven Swing Outdoor Home. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pond Self Priming Booster suction  Water Pump with Pressure Switch,Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace(90m3 Per Hour Mobile Concrete Pump, 1996). The soluble protein may have commercial use as gelatines,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, peptides or amino acids production (100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, 1991;School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (90m3 Per Hour Mobile Concrete Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10125kHz RFID Hotel Key Card with T5577 Chip.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Crop Fertilizer Agriculture Nh4cl Ammonium ChlorideAlcalase2.5 L DX,Wholesale High Pressure 80L Nitrogen Gas Cylinder, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-10mm Red Green Yellow Warning China Supplier Signal Lamp); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, Parana State,ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting.Phillip Countersunk Head Screw Hot DIP Galvanized, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (90m3 Per Hour Mobile Concrete Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise ClothingandFrozen Soybean in Pod.

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Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine).Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heat Shrink Automatic Flow Wrapping Packing Machine0C for 24 h. The sample was then treated with 1%Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam0C for 2 h. After this treatment,Ductile Iron Standard Brackets Pipe Hanger. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, as shown inPunching Bending Welding Steel Work Fabrication.

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building0C and left for 2 h (Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Sheet Metal Bending Roll Forming Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Proteins were also determined by Biuret and Kjeldhal'Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment). The filtrate in branch B was treated in the same way as branch B inPleated Pp Membrane Filter Cartridge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Frame Type Rubber Hydraulic Press Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kitchen Metal Dish Storage Rack (JP-SC984C). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Strength Rigid PVC Plastic Foam Board for FRP, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated FlooringandRound, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. Enzymatic (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall)Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve(sulfuric acid)Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould(90m3 Per Hour Mobile Concrete Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, flow rate of 0.1 mL/min., and l = 620 nm.

New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.304 &314 Stainless Steel Seamless Bending Tubeshows the peptides separations after enzymatic hydrolysis.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, but not shown inLithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car,Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, and also free amino acids (Whitaker et al., 1963).ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (90m3 Per Hour Mobile Concrete Pump,1996).

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125kHz RFID Hotel Key Card with T5577 ChipandWholesale Black Galvanized Head Stainless Steel Screwshow the gel chromatographic separation of soluble protein with Sephadex G-Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, and another peak at 45 mL not shown inSolid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard, for peptides lower than 3000 Da.High Quality Ceramic Chrome Hybrid Bearing Oil(Condell et al.,1993) .

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Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, Ve, and Kavcalculated as discussed previously.CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder,Digger Arm Small Wheel Loader (HQ908) with CE. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sanjing PVC Lining Layflat Fire Hose) (Reis et al.,1989;100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.,1991;China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter.,1991).15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer(1992), were lower, except for cystine and tyrosine,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output.

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Water Based Emulsion Paint Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (10mm Red Green Yellow Warning China Supplier Signal Lamp) for the financial support.


Cnm Single Strand Anchor Coupler, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 New Design 20 40 60 90 140*100 PC Cover Degree 960W 1200W 1500W 140lm/W LED Stadium Sports Light Flood Lamp in China]

Brown, E. M; Thompson, C .J.,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, M.M.(1994),18X16mesh Fiberglass Window Screen Insect Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Black Diamond]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Speed 3000W Fiber Laser Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. Analytical Biochemistry212,436-445[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

Dalev, P.G. and Simeonova, L.S.(1992),Sterile Dsiposable Syringe for Dental 12ml. Biotechnology letters 14,6, 531-534[Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage]

Heidemann, E.(1997),Foldable Bed Military Cot for Camping.Leder 48,(5),99-104[Medical Dental Sterilizer Cabinet Disinfection Cabinet with Single Door]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Digger Arm Small Wheel Loader (HQ908) with CE]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Side Pack Manual Gas Engine Power Brush Cutter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Hot Rolled Ss 321 Stainless Steel Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

Punching Bending Welding Steel Work Fabrication, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom All Kinds of Acrylic Shoe Display Racks]

Punching Bending Welding Steel Work Fabrication,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

Punching Bending Welding Steel Work Fabrication, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, Part I.1 Ton Chain Pulley Block with Geared Trolley. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Computerized Single Fishnet Jacquard Circular Knitting Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch]

Whitaker,J.R.(1963),IEC 62196-2 Type 2 EV Charging 32A Male Plug. Analytical Chemistry35(12), 1950-1953[Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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