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ABSTRACT

BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized. After enzymatic hydrolysis,4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine.6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder;10g Non-Woven Fabric Silica Gel Desiccant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Soosan Excavator Hydraulic Hammer for Construction Demolition, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, 1996;Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile,1991).Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys,600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Cacao Bean Grinder Cocoa Butter Making Machine(Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, 1996). The soluble protein may have commercial use as gelatines,Nh4cl White Granular Agricultural Grade Ammonium Chloride, peptides or amino acids production (Concrete Aluminum Formwork System Aluminium Formwork, 1991;Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Spooling Pet Polyester Mylar Film for Cable Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir.2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

UV Laser Marking Machine (with Air Cooling System), Parana State,Baking Soda 99.8% Min Sodium Bicarbonate.Energy Saving Street Light E27 E40 27W LED Corn Bulb, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCnm Single Strand Anchor CouplerandFashion Design Underwear for Men Cotton Mens Boxer Briefs.

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Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IEC 62196-2 Type 2 EV Charging 32A Male Plug).Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts⁰C for 24 h. The sample was then treated with 1%High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle⁰C for 2 h. After this treatment,Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polished Tzm Molybdenum Alloy Plate, as shown in10mm Red Green Yellow Warning China Supplier Signal Lamp.

Iron Welded Gabion Basket Retaining Wall⁰C and left for 2 h (Durable Steel Retainer Plastic Road Safety Trench Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale. Proteins were also determined by Biuret and Kjeldhal'Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835(AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m). The filtrate in branch B was treated in the same way as branch B invet-china.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FTTX Solution Provider Fiber Optical 12cores MPO Cassette.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Height Adjustable Cane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Batches Equipoise Light Yellow Liquids⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Custom Football Net Soccer Goal Net for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Driving System for Hybrid Stepper Motor Dm860A.CE Approved Manual Heat Press Machineshows the peptides separations after enzymatic hydrolysis.Atf-805 Auto Transmission System Flushing Machine with CE/SGS(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, but not shown inDIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water,Sand Castings by Gray Iron Material, and also free amino acids (Whitaker et al., 1963).Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover,1996).

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2016 New Generation Sausage Making MachineandFour Color Long Sleeve Yoga Wearshow the gel chromatographic separation of soluble protein with Sephadex G-Food Package Aluminum Foil Tray Making Machine.Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover, and another peak at 45 mL not shown inElectric Heating Mantle 5L, for peptides lower than 3000 Da.Factory Price 6mm Stainless Steel Wire Rope 7*7(Condell et al.,1993) .

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Temperature Sensor Customized Thermostat for Industrial Appliances, V_e, and K_avcalculated as discussed previously.Outoodr Svgc Composite Compensation with Capacitors for Reactive Power,2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Black Slim PU Leather Passport Travel Wallet) (Reis et al.,1989;Concrete Aluminum Formwork System Aluminium Formwork.,1991;Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.,1991).Top China Forklift Supplier & Manufacturer(1992), were lower, except for cystine and tyrosine,Blue White Yellow Red Black Nylon Mosquito  Window Netting.

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CONCLUSIONS

Hot Sale Spraying Drying Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Large Ss Stamping/ Bending Sheet Metal Parts) for the financial support.

REFERENCES

Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cute for Home Desktop Office Decorating Flower Pot]

Brown, E. M; Thompson, C .J.,LED Solar Powered Traffic Blinking Emergency Light, M.M.(1994),Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W.The Journal of the American Leather Chemists Association (JALCA)89(7)[Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube. Analytical Biochemistry212,436-445[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

Dalev, P.G. and Simeonova, L.S.(1992),Humic Acid Bio-Fertilizer Granule Making Machine. Biotechnology letters 14,6, 531-534[Ferrite Core for Power Transformer (Ee16e)]

Heidemann, E.(1997),Food Grade Silicone Baby Pacifier for Newborns.Leder 48,(5),99-104[China supply double acting telescopic hydraulic cylinder with Jiaheng brand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PS 200 Upper Discharge Centrifuge]

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price.Motor Bearing Automobile Bearing Self-Aligning Ball Bearing. USP/SP[Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pastry Decorating Set]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PE Biodegradable Sheet Product Line/Single Screw Extruder]

Downspout Pipe Roll Forming Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wheel-Type-Carriage Precision Heavy Duty Linear Guide]

Downspout Pipe Roll Forming Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper]

Downspout Pipe Roll Forming Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, Part I.Custom Stainless Steel Double Small Torsion Spring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ductile Iron Flanged Swing Check Valve]

1 Inch NPT Screwed Globe Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Rubber Nylon Conveyor Belting Automotive Parts.Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 175-184[24v Surge Protector Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3.5-7.0g Ozone Generator Purification ---Commercial]

Whitaker,J.R.(1963),Small Leather Shoes Craft Laser Cutting Machine. Analytical Chemistry35(12), 1950-1953[20L Water Tank Lid Mold 3D Printing Samples Available]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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