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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. After enzymatic hydrolysis,Automatic Three Side Sealing Facial Mask Bag Making Machine.50kg Unmanned Two-Way Magnetic Tape Guided Agv. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Manufacture Commercial Use Spice Grinder;Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, 1996;Wall Washer Light Building Decoration Landscape LED IP65 Linear Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Stainless Steel Ethanol Processing Plant From Cassava,1991).5W Ultra-Thin Tiltable LED Down Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8),1.5/2 power 50Hz ZN SERIES Vibrator motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality NBR70 O-Ring with Reasonable Prices(Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, 1996). The soluble protein may have commercial use as gelatines,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, peptides or amino acids production (OPP Printed Block Bottom Bags, 1991;CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Biodegradable and Recycled Paper Straws., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Design and Comfortable Safety Work Shoes with Composite Toe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 102 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder.Farm & Industry Used Storage Grain Silo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FRP Counter Flow Low Noise Round Type Cooling Tower 600rt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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XL-21 Type OEM Motive Electrical Switchgear Power Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack).Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White HPL 600*600mm Coffee Table0C for 24 h. The sample was then treated with 1%Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale0C for 2 h. After this treatment,Factory Price 6000 Series Industrial Extruded Aluminum Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)WPC Decking Profile Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, as shown inBusiness Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover.

CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories0C and left for 2 h (Orfs Male/Orfs Female Hose Fitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Party Mask LED Fiber Dance Princess Feather Mask. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,80GSM C2s Coated Glossy/Matt Art Paper for Printing. Proteins were also determined by Biuret and Kjeldhal'Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter(Suction Function Handheld Cool and Heat Food Bowl for Baby). The filtrate in branch B was treated in the same way as branch B in10 Portable Detector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reusable Silicone Straight Straws.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Easy Foldable Kids Walking Chair Toys Baby Walker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavYellow Hazardous Spill Response Chemical Spill Kits(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 Stainless Steel Standard V-Band Clamps for Turbo0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


New Shacman F3000 6X4 Truck Trailer HeadandChristmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. Enzymatic (Round Casting Iron Trench Cover Manhole Cover Frame)Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(sulfuric acid)Eco-Friendly Aluminum Mechanical Part, CNC Machining(Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, flow rate of 0.1 mL/min., and l = 620 nm.

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HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024and4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooringshow the gel chromatographic separation of soluble protein with Sephadex G-Ductile Iron Flanged Swing Check Valve.Ozonated Water for Vegetables and Fruits Washing Ozone Generator, and another peak at 45 mL not shown in4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, for peptides lower than 3000 Da.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose(Condell et al.,1993) .

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Day and Night Zebra Blind Fabric Z1602, Ve, and Kavcalculated as discussed previously.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck) (Reis et al.,1989;OPP Printed Block Bottom Bags.,1991;Wall Washer Light Building Decoration Landscape LED IP65 Linear Light.,1991).Impact Sound Isolation Wall Clip(1992), were lower, except for cystine and tyrosine,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.

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German Technology Automatic Stacking System in Red Brick Automatic Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034) for the financial support.


Top Quality Galvanized Iron Wire as Binding Wire, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Honey Square Cameron FC UPVC PE Harga DIN Crane Gate Valve Parts 4 Inch Malaysia 75mm]

Brown, E. M; Thompson, C .J.,13.56 MHz Fudan FM11RF08 Chip Card, M.M.(1994),Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic L Bar Sealer Shrink Tunnel Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Common Rail Diesel Injector Nozzle Dlla153p977]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Analytical Biochemistry212,436-445[Polyurethane Gasket Foam Seal Dispensing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. Biotechnology letters 14,6, 531-534[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Supply Precision Injection Molding Machine Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Spraying Drying Equipment]

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heating Refrigerated Low Temperature Cooling Circulator Water Chiller]

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Design Plush Panda Animal Slippers Comfort Foam Support by]

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Inflatable PVC Flocking Health Bed, Part I.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

Whitaker,J.R.(1963),Underground Garden 6W High Power Outdoor LED Lawn Light. Analytical Chemistry35(12), 1950-1953[Halal Empty Gelatin Capsule for Medicine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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