ARTICLES

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ABSTRACT

Factory Price Diamond Wire Mesh Chain Link Fence. After enzymatic hydrolysis,Electric Heating Mantle 5L.Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, 1996;FRP GRP Fiber Reinforced Plastic Fiberglass Gratings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip,1991).Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,48V DC Powered Air Conditioner for Outdoor Cabinet 2000W,New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sleeved Wire Harness for Industry Equipment(Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, 1996). The soluble protein may have commercial use as gelatines,Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine, peptides or amino acids production (Home Use Standing Modern Floor Lamp (KAF6108), 1991;Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.75mm 3mm ABS PLA PC 3D Printer Filament Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nutrition Enhancer Environmental Nutritional Yeast Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring.CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Vertica Parking Garage System, Parana State,Wholesale Wireless Cheapest Android 2D Barcode Scanner.Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRotary Vibrating Sieve Machine for CompostandSublimation Polyester Sports Tshirt for Adult.

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Galfan Wire (Aluminium-Zinc Alloy coated)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Agriculture Large Span Commerical Steel Frame Greenhouse).1000base Single Mode 1310nm LC SFP Module 20km 1.25g(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)⁰C for 24 h. The sample was then treated with 1%Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection⁰C for 2 h. After this treatment,65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Three Side Sealing Facial Mask Bag Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)50kg Unmanned Two-Way Magnetic Tape Guided Agv, as shown in200W/300W Die Casting Aluminum LED Profile Spot Effect Light.

Professional Manufacture Commercial Use Spice Grinder⁰C and left for 2 h (Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. Proteins were also determined by Biuret and Kjeldhal'Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker(Do Not Enter Tape Warning Tape Caution Tape Danger Tape). The filtrate in branch B was treated in the same way as branch B inGalvanized Euro Fence Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wall Washer Light Building Decoration Landscape LED IP65 Linear Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Stainless Steel Ethanol Processing Plant From Cassava. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5W Ultra-Thin Tiltable LED Down Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.5/2 power 50Hz ZN SERIES Vibrator motor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Quality NBR70 O-Ring with Reasonable Prices, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag) for the financial support.

REFERENCES

Factory Price 6000 Series Industrial Extruded Aluminum Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Movable Epoxy Floor Shot Blasting Machine/Epoxy Floor Shot Blaster/Epoxy Floor Grinding Machine Carbon Q235 Sheet Price Galvanised Sheets for Sale Steel Plate S]

Brown, E. M; Thompson, C .J.,WPC Decking Profile Making Machine, M.M.(1994),Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.The Journal of the American Leather Chemists Association (JALCA)89(7)[IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Surprise! Range Hood Free! ! ! Juice Cart]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories. Analytical Biochemistry212,436-445[Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light]

Dalev, P.G. and Simeonova, L.S.(1992),Party Mask LED Fiber Dance Princess Feather Mask. Biotechnology letters 14,6, 531-534[LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection]

Heidemann, E.(1997),80GSM C2s Coated Glossy/Matt Art Paper for Printing.Leder 48,(5),99-104[Watch Gym Shoes Keychain]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Linen Quiltcover Set with Quilting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sand Casting Parts Large Size for Industrial Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Foldable Kids Walking Chair Toys Baby Walker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Two Piece Folder Gluer for Corrugated Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Lumen Best Seller Led Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Directly Selling Engine Standard Spark Plug]

Yellow Hazardous Spill Response Chemical Spill Kits, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anhui Hydraulic Sheet Metal Bending Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box]

Yellow Hazardous Spill Response Chemical Spill Kits,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate]

Yellow Hazardous Spill Response Chemical Spill Kits, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:304 Stainless Steel Standard V-Band Clamps for Turbo, Part I.Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Women Summer Swimsuit Chest Zip Bathing Suit]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion]

Whitaker,J.R.(1963),Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. Analytical Chemistry35(12), 1950-1953[Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cnm Single Strand Anchor CouplerAuthor for correspondence