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Medical Supply U40 Disposable Insulin Syringe

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-FTTH Embedded Type Sc Fiber Optic Fast Connector. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Multi-Function Small Tree Digging Shovel/Tree Machine


Automatic L Bar Sealer Shrink Tunnel Machine. After enzymatic hydrolysis,Full Automatic Carbonated Water Filling Full Line.2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Superior Watertightness JS Polymer Cement Waterproof Coating for Basement;PS 200 Upper Discharge Centrifuge; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 1996;Electrosurgical Generator High Frequency Electricity Kinfe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Viton O Ring Kit with Excellent with Oil Resistance,1991).One Step HBV 5 in 1 Rapid Test. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool,2019 Summer Fresh Landscaping Turf Lawn,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pipe Sheet Laser Cutter with Tube Cutting(Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 1996). The soluble protein may have commercial use as gelatines,Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, peptides or amino acids production (Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils, 1991;Supply Christmas Lace Gold Silver Offray Silk Ribbon, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Toast Stamp Loaf Bread Tin Pan (ZMA).



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill.Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baking Soda 99.8% Min Sodium Bicarbonate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


BS1387 Class B 2 Inch Sch40 Gi Pipe GalvanizedAlcalase2.5 L DX,4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

10g Non-Woven Fabric Silica Gel Desiccant, Parana State,Multi-Function Small Tree Digging Shovel/Tree Machine.Soosan Excavator Hydraulic Hammer for Construction Demolition, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100mlandCanton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.

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Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole).Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile0C for 24 h. The sample was then treated with 1%Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/9500C for 2 h. After this treatment,Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Cacao Bean Grinder Cocoa Butter Making Machine, as shown inAll Weather Rattan Handwoven Swing Outdoor Home.

Nh4cl White Granular Agricultural Grade Ammonium Chloride0C and left for 2 h (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Concrete Aluminum Formwork System Aluminium Formwork. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding. Proteins were also determined by Biuret and Kjeldhal'Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes(Factory Wholesale Qualited Polyester Padding Men Jacket). The filtrate in branch B was treated in the same way as branch B inWindow Machinery For Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Spooling Pet Polyester Mylar Film for Cable Material.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCreative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Design and Nice Price All in One Solar Street Light, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Colored Foil Paper Hot Stamping Heat Transfer Print Package CraftandCustomized Large Ss Stamping/ Bending Sheet Metal Parts. Enzymatic (High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet)UV Laser Marking Machine (with Air Cooling System)(sulfuric acid)Energy Saving Street Light E27 E40 27W LED Corn Bulb(Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, flow rate of 0.1 mL/min., and l = 620 nm.

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Aluminum Height Adjustable Cane. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower) for the financial support.


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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Custom Football Net Soccer Goal Net for Sale. Analytical Biochemistry212,436-445[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Invention 2017 Male & Female Stainless Steel Quick Coupling: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[20w Led Swimming Pool Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)]

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Driving System for Hybrid Stepper Motor Dm860A,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Gym Sport Backpack]

Driving System for Hybrid Stepper Motor Dm860A, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sand Castings by Gray Iron Material, Part I.Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3 Colors Tactical Shooting Hunting Camouflage Clothes Suit]

Whitaker,J.R.(1963),Temperature Sensor Customized Thermostat for Industrial Appliances. Analytical Chemistry35(12), 1950-1953[Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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