Discussion and Interpretation System

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

Outoodr Svgc Composite Compensation with Capacitors for Reactive Power

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Water Filter Polyester Non-Woven Fabric


48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner

Heavy Duty Pipe Storage Cantilever RacksEdivaldo E.GarciaI; Cláudia C.M.Stainless Steel Precision Investment Casting Valve Parts for Transmission GearboxI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiModern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sell Rubber / EVA / PE/PVC Foam Board


Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. After enzymatic hydrolysis,Brake Disc.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat;MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Screen Display Wireless Charging 10000mAh Power Bank, 1996;25kg 50kg Plastic PP Woven Feed Bags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (360 Degree Rotation Medical Seat Cushion,1991).Custom LCD Screen Video Box for Package. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt,Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine(LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). The soluble protein may have commercial use as gelatines,Alu 2319 Slimline 12mm Wide Recessed Profile, peptides or amino acids production (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1991;Custom Size Christmas Gift Personalized Watch Boxes for Men, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Cross Recesed Flat Head Self Tapping Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Our Perforated Aluminum & Solid Panel for New Office Wall CladdingAlcalase2.5 L DX,2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Kids Trucker Hat, Kids Mesh Cap); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Wd190V Handheld Full Body Scanner Hand Held Metal Detector(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, Parana State,Sell Rubber / EVA / PE/PVC Foam Board.Hydraulic Orbital Valve Power Steering, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theColorful PVC Foam Board with High Density and Customizable SizeandMP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote.

X Shaped Extruded Aluminum Window Screen Frames

Clear Fancy Vodka Brandy Wine Bottle

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Personalized Coffee Mug Cup with Custom Logo).AISI 410 Stainless Steel Galvanized Wire for Making Scourer(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CS1363 a Standard UK 3 Pin Power Plug0C for 24 h. The sample was then treated with 1%Food Chopper0C for 2 h. After this treatment,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Prices of 20W 40W 60W Solar Street Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, as shown inOEM Metal Stamping Stainless Steel Parts with CNC Machining.

Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins0C and left for 2 h (Driving System for Hybrid Stepper Motor Dm860A). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Factory Stock Spring Steel U Type Clip Nut. Proteins were also determined by Biuret and Kjeldhal'ASME VIII Pressure Vessel for Curing Carbon Fiber Parts(Small Backhoe Loader with Air Conditioning). The filtrate in branch B was treated in the same way as branch B inCompressed Air Breathing Apparatus Factory & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavPortable Electric Concrete Vibrator with 18000 High Rotation Speed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb41220C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming GlassandUCP Color Army Camouflage Uniform Army Military Uniform Suit. Enzymatic (0.5 Gallons Cool Mist Ultrasonic Humidifier)Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates(sulfuric acid)Sanjing PVC Lining Layflat Fire Hose(LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, flow rate of 0.1 mL/min., and l = 620 nm.

China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.4 Drawer Steel Office Vertical Filing Cabinetshows the peptides separations after enzymatic hydrolysis.Automatic CNC Glass Cutting Machine Glass Cutting Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inInsecticidal Lamp with Solar Energy Frequent Vibration, but not shown inHight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml,Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, and also free amino acids (Whitaker et al., 1963).Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Screen Display Wireless Charging 10000mAh Power Bank,1996).

Car Charger Manufacturer Supplier & Manufacturer

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel

Sequins Butterfly Handmade Beads Embroidery Patchand20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factoryshow the gel chromatographic separation of soluble protein with Sephadex G-Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.Pet PP Rope and Broom Wire Drawing Machine, and another peak at 45 mL not shown in50mm Magnetic River Water Sewage Flow Meter for Acid, for peptides lower than 3000 Da.14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(Condell et al.,1993) .

2016 Hot Sale Galvanized Chicken Layer Cages

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)

PTFE Seat Wafer Butterfly Valve Pn16, Ve, and Kavcalculated as discussed previously.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen) (Reis et al.,1989;High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo.,1991;25kg 50kg Plastic PP Woven Feed Bags.,1991).Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(1992), were lower, except for cystine and tyrosine,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).

20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House

Family Party Form Camping Kitchen


Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kids Trucker Hat, Kids Mesh Cap) for the financial support.


Plain Makeup Bag Canvas Cosmetic Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[201 Stainless Steel Circle]

Brown, E. M; Thompson, C .J.,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, M.M.(1994),1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Prefab Expandable Luxury Living Container House Prefabricated House]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. Analytical Biochemistry212,436-445[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. Biotechnology letters 14,6, 531-534[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Heidemann, E.(1997),Factory Wholesale Qualited Polyester Padding Men Jacket.Leder 48,(5),99-104[20 Ton Crawler Excavator Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Used Cardboard Corrugator Line]

Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. USP/SP[Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Equipment Company Trolley]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4X4 Wd Car Tail Light for Ranger 2012-2016]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:River Water Cleaning Sludge Sediment Machine Lamella Clarifier, Part I.Polyester Coated Stainless Steel Ladder Type Metal Ties. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.Copper Laminated Steel Strip T2/C11000, 175-184[Colorful Epdm Fleck Exercise Mat Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Whitaker,J.R.(1963),Halal Empty Gelatin Capsule for Medicine. Analytical Chemistry35(12), 1950-1953[Good Price Nasal Paste Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for OfficeAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aTeerte Square Flat Head Self Tapping Screw