ARTICLES

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ABSTRACT

Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. After enzymatic hydrolysis,Brake Disc.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat;MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Screen Display Wireless Charging 10000mAh Power Bank, 1996;25kg 50kg Plastic PP Woven Feed Bags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (360 Degree Rotation Medical Seat Cushion,1991).Custom LCD Screen Video Box for Package. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt,Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine(LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). The soluble protein may have commercial use as gelatines,Alu 2319 Slimline 12mm Wide Recessed Profile, peptides or amino acids production (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1991;Custom Size Christmas Gift Personalized Watch Boxes for Men, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Screen Display Wireless Charging 10000mAh Power Bank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Cross Recesed Flat Head Self Tapping Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Personalized Coffee Mug Cup with Custom Logo).AISI 410 Stainless Steel Galvanized Wire for Making Scourer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CS1363 a Standard UK 3 Pin Power Plug⁰C for 24 h. The sample was then treated with 1%Food Chopper⁰C for 2 h. After this treatment,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Prices of 20W 40W 60W Solar Street Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, as shown inOEM Metal Stamping Stainless Steel Parts with CNC Machining.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPortable Electric Concrete Vibrator with 18000 High Rotation Speed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kids Trucker Hat, Kids Mesh Cap) for the financial support.

REFERENCES

Plain Makeup Bag Canvas Cosmetic Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[201 Stainless Steel Circle]

Brown, E. M; Thompson, C .J.,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, M.M.(1994),1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Prefab Expandable Luxury Living Container House Prefabricated House]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. Analytical Biochemistry212,436-445[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. Biotechnology letters 14,6, 531-534[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Heidemann, E.(1997),Factory Wholesale Qualited Polyester Padding Men Jacket.Leder 48,(5),99-104[20 Ton Crawler Excavator Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Used Cardboard Corrugator Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Equipment Company Trolley]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4X4 Wd Car Tail Light for Ranger 2012-2016]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:River Water Cleaning Sludge Sediment Machine Lamella Clarifier, Part I.Polyester Coated Stainless Steel Ladder Type Metal Ties. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Whitaker,J.R.(1963),Halal Empty Gelatin Capsule for Medicine. Analytical Chemistry35(12), 1950-1953[Good Price Nasal Paste Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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