ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Safety Double Wire Security Metal Welded Wire Mesh Fence. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pneumatic Air Driven Liquid Booster Pump

ABSTRACT

Heavy Duty Wood Door Calibrating Wide Belt Sander. After enzymatic hydrolysis,Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women Summer Swimsuit Chest Zip Bathing Suit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hemp Seed Oil Body Lotion Moisturizes The Skin, 1996;SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal,1991).UTP CAT6 48 Ports Vertical Termination Patch Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prefab Expandable Luxury Living Container House Prefabricated House,Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries(Hemp Seed Oil Body Lotion Moisturizes The Skin, 1996). The soluble protein may have commercial use as gelatines,Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, peptides or amino acids production (Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, 1991;Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Must 4kw 2HP Pump Power Inverter Ags Bts Available.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Small Steel Silo for Poultry Feed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hemp Seed Oil Body Lotion Moisturizes The Skin, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Price Nasal Paste Packaging Machine.Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen, Parana State,Pneumatic Air Driven Liquid Booster Pump.OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hemp Seed Oil Body Lotion Moisturizes The Skin, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial Activated Carbon Water Filter Industry Water TreatmentandModern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.

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Ms Gi Pipe Dn15~Dn400 Gi Pipe List(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brass Pump Spare Parts for Circulating Pump).Low-E Insulating Glass Curtain Wall(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024⁰C for 24 h. The sample was then treated with 1%Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)⁰C for 2 h. After this treatment,Fashion Design Underwear for Men Cotton Mens Boxer Briefs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nylon Balance Bike with Water Transfer Printing Malaysia's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti Slip Rubber Mat, as shown inInterior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.

5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap⁰C and left for 2 h (Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair. Proteins were also determined by Biuret and Kjeldhal'Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml(750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish). The filtrate in branch B was treated in the same way as branch B inChina V Buckle Swimwear. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automotive Perforated Metal Stamping Part for Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Car Accessories Seat for Child(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zipper Pouch Pistachio Nuts Premade Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight) for the financial support.

REFERENCES

Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3d Cartoon Nail Art Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen, M.M.(1994),DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Two Piece Folder Gluer for Corrugated Box. Analytical Biochemistry212,436-445[Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry]

Dalev, P.G. and Simeonova, L.S.(1992),British Standard 2 Gang 1 Way Wall Light Switch. Biotechnology letters 14,6, 531-534[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

Heidemann, E.(1997),Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts.Leder 48,(5),99-104[Portable Air Purifier HEPA Filter for Personal Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1500nit Outdoor Touch Monitor Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, Part I.Rectangular Stainless Steel Pipe SS304. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6.6kw LiFePO4 Charger for Lithium Battery Pack.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, 175-184[World First Inline Pick and Place Machine for 1200mm LED]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Foldable Bed Military Cot for Camping]

Whitaker,J.R.(1963),50m Depth Long Range Metal Detector with Reliable Quality. Analytical Chemistry35(12), 1950-1953[Heavy Duty Wood Door Calibrating Wide Belt Sander]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Safety Double Wire Security Metal Welded Wire Mesh FenceAuthor for correspondence