ARTICLES

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ABSTRACT

Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. After enzymatic hydrolysis,Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush.Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Manufacturer Industrial Aluminum Floor Bar Grating;Food Grade Stainless Steel Milk Dairy Equipment for Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1996;Low Frequency 3000W 24V 230V Inverter with Battery Charger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amazon Best Selling Sports Ankle Support Sleeves Compression,1991).Pen Type pH Meter pH-031&0.00-14.00pH Tester. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8mm Heavy Duty Nails U Shaped Galvanized Staples,ASTM Iron Sand Casting Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,200W/300W Die Casting Aluminum LED Profile Spot Effect Light(Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1996). The soluble protein may have commercial use as gelatines,2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X, peptides or amino acids production (Ce Approved Sperm Concentration Test for Sperm Density Test, 1991;Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sand Casting Parts Large Size for Industrial Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Durable Steel Retainer Plastic Road Safety Trench Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Iron Cast Brake Drums for Trucks.Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Folding Aluminium Easy Move Choral Stage with Wheels for School(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, Parana State,Small Leather Shoes Craft Laser Cutting Machine.Milk Cream Disc Centrifugal Separator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Selling Military Large Event Marquee Tent Saleand4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall.

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Bobcat Skid Steer Loader Bucket Teeth 6737325lp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Modern Vertica Parking Garage System).Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Counter Top Working Table Chiller⁰C for 24 h. The sample was then treated with 1%Cashew Grading Machine for Cashew Processing Line⁰C for 2 h. After this treatment,4X4 Wd Car Tail Light for Ranger 2012-2016. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings, as shown inLuxury Aroma Reed Diffuser with Rattan Sticks for Decoration.

Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera⁰C and left for 2 h (Color Cardstock Color Paper Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Power Press Perforated Metal Punch Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100%Polyester Satin Fabric for Blouse. Proteins were also determined by Biuret and Kjeldhal'Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure(Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag). The filtrate in branch B was treated in the same way as branch B inStainless Steel Wok with Soft Touch Handle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High-End Compression Rash Guards, Long Sleeve Compression Shirt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/9~12 Inch Mechanical Backing Plate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN929 Low-Cost Supply Hexagon Weld Nut, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manual Sealing Machine for Instant Noodles Paper Soup BowlsandDecoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. Enzymatic (High Efficiency Solar Cell Photovoltaic 300W Hot Sell)60m3/H Lamella Clarifier for River Water Treatment(sulfuric acid)SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)(Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxesshows the peptides separations after enzymatic hydrolysis.Soldering Machine LED Module PCB Hot Air Soldering Station(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, but not shown inBaijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring,220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist, and also free amino acids (Whitaker et al., 1963).Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump,1996).

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Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, V_e, and K_avcalculated as discussed previously.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK,X-Humate 95% Min Super Sodium Humate (Nut Moradant). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Gym Sport Backpack) (Reis et al.,1989;Ce Approved Sperm Concentration Test for Sperm Density Test.,1991;Low Frequency 3000W 24V 230V Inverter with Battery Charger.,1991).Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board(1992), were lower, except for cystine and tyrosine,Women Summer Swimsuit Chest Zip Bathing Suit.

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CONCLUSIONS

Hemp Seed Oil Body Lotion Moisturizes The Skin. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor) for the financial support.

REFERENCES

SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Precision Zirconia Zro2 Ceramic Ball]

Brown, E. M; Thompson, C .J.,Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, M.M.(1994),UTP CAT6 48 Ports Vertical Termination Patch Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Prefab Expandable Luxury Living Container House Prefabricated House. Analytical Biochemistry212,436-445[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box. Biotechnology letters 14,6, 531-534[New Invention 2017 Male & Female Stainless Steel Quick Coupling]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention]

Industrial Rubber Nylon Conveyor Belting Automotive Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close.Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization. USP/SP[8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automotive Bearings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Hexagonal Wire Mesh Gabion / Gabion Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cbd Vape Oil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags]

10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Must 4kw 2HP Pump Power Inverter Ags Bts Available-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sell Rubber / EVA / PE/PVC Foam Board]

10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut]

10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Small Steel Silo for Poultry Feed, Part I.Good Price Nasal Paste Packaging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical Type Caseformer Machine WJ-LKX-15]

Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 175-184[Dn400--3000 New Product Plastic HDPE Irrigation Drain Pipe High Pressure Drinking Water HDPE Carat Pipe Krah Spiral Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[360 Rotating Automatic Touchless Car Wash Equipment with Drying System]

Whitaker,J.R.(1963),Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate. Analytical Chemistry35(12), 1950-1953[Building Material High Quality 316 Stainless Steel Grating]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

X-Humate 95% Min Super Sodium Humate (Nut Moradant)Author for correspondence