ARTICLES

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ABSTRACT

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. After enzymatic hydrolysis,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags.PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, 1996;Factory Directly Selling Engine Standard Spark Plug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Logo Silicone Wristband, Rubber Wrist Bands,1991).Custom All Kinds of Acrylic Shoe Display Racks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide,Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Supply U40 Disposable Insulin Syringe(Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, 1996). The soluble protein may have commercial use as gelatines,Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, peptides or amino acids production (Water Chiller Air Cooled Chiller Industrial Chiller for Sale, 1991;Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Precision Tungsten Carbide Machine Customized Cutting Tool., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Maleic Anhydride CAS 108-31-6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2000mj Q Switched for Pigment Removal with Ce Certification.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cow Dung Chicken Manure Organic Fertilizer Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes).Industrial Activated Carbon Water Filter Industry Water Treatment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2016 New Generation Sausage Making Machine⁰C for 24 h. The sample was then treated with 1%Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)⁰C for 2 h. After this treatment,Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Supplier Building Construction Steel Frame (ZY431), as shown in10ton Telescopic Hydraulic Truck Crane.

iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing⁰C and left for 2 h (Rainsky Ceiling Mounted Stainless Steel Shower Heads). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lab Equipment portable Fume Hood. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt. Proteins were also determined by Biuret and Kjeldhal'10ton Telescopic Hydraulic Truck Crane(Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts). The filtrate in branch B was treated in the same way as branch B inCe Solar Energy Manufacturers Decorative Outdoor Flashlight LED Garden Light Dancing LED Touch Light LED Flame Light Solar Lighting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCracker Multi Rows Trayless Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2 Quick Change Flexible Roloc Disc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Aluminium Windows Thermal Break China Supplier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Low Power Consumption Laser Foam Vertical Die Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20kv 50kVA High Voltage Dry Type Power Supply Transformer) for the financial support.

REFERENCES

New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6q0945511]

Brown, E. M; Thompson, C .J.,Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, M.M.(1994),Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211). Analytical Biochemistry212,436-445[Low Power Consumption Laser Foam Vertical Die Cutting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. Biotechnology letters 14,6, 531-534[Six Stations Pen Press Machine]

Heidemann, E.(1997),High Quality Crusher for Used Plastic Recycling Line.Leder 48,(5),99-104[Smart Water Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning]

Valve Bag Dry Mortar Bagging Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit.SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. USP/SP[Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wear Resitant Rope End Cap Plastic Flange Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Interior Fiber Cement Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Comfortable Two Piece Women Fitness Clothing for Gym Wear, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fire Extinguisher UV Curing Machine Cylinder Drying Machine]

Comfortable Two Piece Women Fitness Clothing for Gym Wear,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[700mm RP HP UHP High Quality Graphite Electrode]

Comfortable Two Piece Women Fitness Clothing for Gym Wear, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flat Surface Mounted 300mm 24W LED Panel Light for Interior, Part I.Latex Paint Emulsion Thickener Agents with High Leveling Property. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Betaine 98% Betaina Feed Grade]

Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Orfs Male/Orfs Female Hose Fitting.Ss400 Galvanized Steel C Channel Steel Price, 175-184[Garnet Magnetic Separator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine]

Whitaker,J.R.(1963),Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping. Analytical Chemistry35(12), 1950-1953[Vertical Automatic Granule Bag Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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