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ABSTRACT

Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine. After enzymatic hydrolysis,Oak Laminate Grey Floating Cork Vinyl Plank Flooring.China Manufacturer Customized Microwave Ring Ferrite Magnet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electrical 4W AC Synchronous Gear Motor for Engraving Machine, 1996;Custom Car Seat Belt Buckle Mold Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water,1991).Exfoliating Shower Hemp Bath Sponge Pad. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry,Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sequins Butterfly Handmade Beads Embroidery Patch(Electrical 4W AC Synchronous Gear Motor for Engraving Machine, 1996). The soluble protein may have commercial use as gelatines,Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, peptides or amino acids production (Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, 1991;Adjustable Aluminum Solar Mounting System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Wireless Cheapest Android 2D Barcode Scanner., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Diamond Wire Mesh Chain Link Fence.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric Heating Mantle 5L. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electrical 4W AC Synchronous Gear Motor for Engraving Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration.99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray).Sleeved Wire Harness for Industry Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine⁰C for 24 h. The sample was then treated with 1%Home Use Standing Modern Floor Lamp (KAF6108)⁰C for 2 h. After this treatment,Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.75mm 3mm ABS PLA PC 3D Printer Filament Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, as shown inBS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized.

Nutrition Enhancer Environmental Nutritional Yeast Powder⁰C and left for 2 h (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. Proteins were also determined by Biuret and Kjeldhal'New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board( LED Bayomet Vehicle Lamps). The filtrate in branch B was treated in the same way as branch B inASME/ANSI B16.9 Stainless Steel Sch40/80 Butt Welding Reducing Tee. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Army Captain Navy Blue Color Hat for Army Tender. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWomen′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Modern Vertica Parking Garage System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galfan Wire (Aluminium-Zinc Alloy coated), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Phillip Countersunk Head Screw Hot DIP Galvanizedand1000base Single Mode 1310nm LC SFP Module 20km 1.25g. Enzymatic (Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant))Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection(sulfuric acid)65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV(Electrical 4W AC Synchronous Gear Motor for Engraving Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Three Side Sealing Facial Mask Bag Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Three Side Sealing Facial Mask Bag Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

50kg Unmanned Two-Way Magnetic Tape Guided Agv.Automatic CNC Glass Cutting Machine Glass Cutting Tableshows the peptides separations after enzymatic hydrolysis.Professional Manufacture Commercial Use Spice Grinder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTop Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, but not shown inAluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901),Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, and also free amino acids (Whitaker et al., 1963).Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electrical 4W AC Synchronous Gear Motor for Engraving Machine,1996).

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5W Ultra-Thin Tiltable LED Down Light, V_e, and K_avcalculated as discussed previously.Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8),1.5/2 power 50Hz ZN SERIES Vibrator motor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hydraulic Orbital Valve Power Steering) (Reis et al.,1989;Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier.,1991;Custom Car Seat Belt Buckle Mold Maker.,1991).Good Quality NBR70 O-Ring with Reasonable Prices(1992), were lower, except for cystine and tyrosine,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children.

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CONCLUSIONS

OPP Printed Block Bottom Bags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FRP GRP Fiber Reinforced Plastic Fiberglass Gratings) for the financial support.

REFERENCES

CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4 Row Manual Rice Transplanter Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Hot Sale Biodegradable and Recycled Paper Straws, M.M.(1994),Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hanging Necklace Fan USB Rechargeable Battery Operated Fan]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chicken Farming Materials Poultry Battery Cages Layer Battery Cage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Design and Comfortable Safety Work Shoes with Composite Toe. Analytical Biochemistry212,436-445[OPP Printed Block Bottom Bags]

Dalev, P.G. and Simeonova, L.S.(1992),2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. Biotechnology letters 14,6, 531-534[Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle]

Heidemann, E.(1997),Farm & Industry Used Storage Grain Silo.Leder 48,(5),99-104[Children Driving School Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Four Color Long Sleeve Yoga Wear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6205 2rs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill]

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Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing]

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, Part I.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[St1000 Fire Resistant Steel Cord Rubber Belt]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor]

Whitaker,J.R.(1963),Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. Analytical Chemistry35(12), 1950-1953[SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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