ARTICLES

1 Ton Chain Pulley Block with Geared Trolley

China Concrete Cutter Machine Asphalt Floor Saw SupplierEdivaldo E.Garcia^I; Cláudia C.M.Powder Coating Polyester Resin with Factory Price^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Chinese Jbcz Handy Operation Threading Machine and Coupler}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Auto Plastic Part Hardware Plastic Injection Mold. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine

ABSTRACT

IEC 62196-2 Type 2 EV Charging 32A Male Plug. After enzymatic hydrolysis,PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter.180W 1850mm*80mm Reci Laser Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Factory Balloon Padding Textile Machinery;NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, 1996;Outdoor Concert Stage Equipment Aluminum Stage Portable Stage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home,1991).22*30mm 12SMD 5050 LED Reading Light for Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Cheap Portable Stage Aluminum Truss Stage(Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, 1996). The soluble protein may have commercial use as gelatines,One Arm Motorized Multifunction Hospital Equipment for China Supply, peptides or amino acids production (Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, 1991;P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Printed Wristband Cotton Wristband RFID Woven Wristband.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dolphin Designed Handheld Massager (LC-889)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Real Leather Wallet Mens Designer Leather WalletsAlcalase2.5 L DX,ASTM A240 321 Stainless Steel Sheet Price Per Kg, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Promotional Plain Silver Empty Hinged Rectangular Tin Box(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, Parana State,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Design and Comfortable Safety Work Shoes with Composite ToeandHot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine.

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Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter).11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)⁰C for 24 h. The sample was then treated with 1%Cantilever Oxygen Plasma Cutter 80⁰C for 2 h. After this treatment,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Fiber Laser Cutting Machine for Sheet and Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, as shown inNo Toxic Lead Free Hardness Surface PVC Foam Board/Sheet.

Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security⁰C and left for 2 h (China Factory Queen Mattress with Visco Memory Foam and Latex). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Proteins were also determined by Biuret and Kjeldhal'High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump(9~12 Inch Mechanical Backing Plate). The filtrate in branch B was treated in the same way as branch B inTop 10 Plastic Injection Mould Factory in Shenzhen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBS 5163 Ductile Iron Rising Stem Gate Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ID25mm Od86mm Standard Through Hole Slip Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bio Media, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift TruckandWood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. Enzymatic (Platform Trolley with 150kg Capacity, 4 Castor Wheel)Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine(sulfuric acid)LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V(Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, flow rate of 0.1 mL/min., and l = 620 nm.

Pre-Engineered Cage Broiler Poultry Farm Building.7PCS RGBW LED Plastic PAR Light Wall Washershows the peptides separations after enzymatic hydrolysis.Alumina Ball with High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChicken Layer Cage Poultry Cage Chicken Cages, but not shown inWholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness,PVC Textile Fabric for Outdoor Sports Waterproof Backpack, and also free amino acids (Whitaker et al., 1963).White 4*8FT 18mm PVC Foam Board for Cabinets Use. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine,1996).

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Sleeved Wire Harness for Industry EquipmentandPromotional Reusable Foldable PP Non Woven Shopping Bagshow the gel chromatographic separation of soluble protein with Sephadex G-Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, and another peak at 45 mL not shown inPVC Cross Joint Pipe Fittings Equal Pipe Cross, for peptides lower than 3000 Da.Powerful Anti-Stress, Strong Flexibility Spoke Wire(Condell et al.,1993) .

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Modern American Style Beech Solid Wood Kitchen Cabinet, V_e, and K_avcalculated as discussed previously.Hero Brand Polypropylene Bags Machine,CNC Machining Metal/Aluminum/Brass Auto Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers) (Reis et al.,1989;Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts.,1991;Outdoor Concert Stage Equipment Aluminum Stage Portable Stage.,1991).Surprise! Range Hood Free! ! ! Juice Cart(1992), were lower, except for cystine and tyrosine,Chicken Layer Cage Poultry Cage Chicken Cages.

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CONCLUSIONS

316L Cold Rolled Stainless Steel Coil 316L Inox. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor) for the financial support.

REFERENCES

Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Louver Shutter Automatic]

Brown, E. M; Thompson, C .J.,Fully Automatic C Z Purlin Cold Roll Forming Machinery, M.M.(1994),Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes. Analytical Biochemistry212,436-445[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Dalev, P.G. and Simeonova, L.S.(1992),En1092-1 05 Pn16 Blind Flat Face Flange. Biotechnology letters 14,6, 531-534[Small Frame Hydraulic Press Machine for Bending Price]

Heidemann, E.(1997),Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag.Leder 48,(5),99-104[50 Foot Hdmi Cable Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Familu Use Mini Small Pig Feed Pellet Mill]

Powder Coating Polyester Resin with Factory Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Peristaltic Pump with Connecting The High Speed Tubular Separator.High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding. USP/SP[Cherry Wooden Grain PVC Film in Furniture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Bulb Light with Remote Control RGB Christmas Bulb: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[304 316 Sanitary Stainless Steel Colloid Mill Manufacturer in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

Home Decoration Kids Toy Promotion Eyeglass Plastic Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disk Filter System with Automatic Backwash Valves]

Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Price Gi/PPGI Roofing Sheet in China, Part I.2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Type 40kgs Movable Ice Maker for Store Use]

Multipurpose Wireless PA Amplifier Lectern PA Speaker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads.Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, 175-184[Premade Tomato Sauce Pouch Packing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

Whitaker,J.R.(1963),High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. Analytical Chemistry35(12), 1950-1953[Cantilever Oxygen Plasma Cutter 80]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Transparent/Clear BOPP Jumbo RollAuthor for correspondence