ARTICLES

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ABSTRACT

Mango Drying Machine/Solar Fruit Drying Machine. After enzymatic hydrolysis,Mineral Industry Magnet Ore Ball Milling Machine.DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel;Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Blasting Cleaning Shot Peeling Machine Tumble Belt Type, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 1996;Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chemical Plant Transfer Industrial Water Pump,1991).Insecticidal Lamp with Solar Energy Frequent Vibration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286,Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Square Washer with Timber Constructions, DIN436DIN436(B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 1996). The soluble protein may have commercial use as gelatines,Do Not Enter Tape Warning Tape Caution Tape Danger Tape, peptides or amino acids production (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), 1991;Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality PVC Plastic Ceiling for Wall Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Steel Structures for Power, Cement, Coal Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+.CE Approved Manual Heat Press Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Crusher for Used Plastic Recycling Line).Nbm High Quality Auto Part Factory Price 6pk2310(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ultrasonic Automatic Face Mask Blank Making Machine⁰C for 24 h. The sample was then treated with 1%Manufacturer of Soda Ash Dense 99 2% Price⁰C for 2 h. After this treatment,Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Headband Big Headphone EVA Bag Tool Case Specialized Storage Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chicken Slaughtering Machine for Sale, as shown inPVC Textile Fabric for Outdoor Sports Waterproof Backpack.

750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish⁰C and left for 2 h (Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hf Vacuum Dryer Machine with Advanced High Frequency Technology. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers. Proteins were also determined by Biuret and Kjeldhal'Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell(Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer). The filtrate in branch B was treated in the same way as branch B inShort Sleeve Casual Shirt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Customized Microwave Ring Ferrite Magnet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nbm High Quality Auto Part Factory Price 6pk2310andPrinting Adhesive Waterproof Custom Membrane Switch for Electric Cooker. Enzymatic (Electrical 4W AC Synchronous Gear Motor for Engraving Machine)Custom Car Seat Belt Buckle Mold Maker(sulfuric acid)DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water(B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Exfoliating Shower Hemp Bath Sponge Pad, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Exfoliating Shower Hemp Bath Sponge Pad, flow rate of 0.1 mL/min., and l = 620 nm.

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Factory Price Diamond Wire Mesh Chain Link Fence, V_e, and K_avcalculated as discussed previously.Electric Heating Mantle 5L,Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bto 22 Electric Galvanized Razor Concertina Wire for Military) (Reis et al.,1989;CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).,1991;Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap.,1991).99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate(1992), were lower, except for cystine and tyrosine,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight.

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CONCLUSIONS

Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner) for the financial support.

REFERENCES

Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Passion Fruit Washing Machine Parallel Washing Machine High Pressure Spray Cleaner Brush Fruit Washing Machine (TS-X300M)]

Brown, E. M; Thompson, C .J.,FRP GRP Fiber Reinforced Plastic Fiberglass Gratings, M.M.(1994),Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Paint Sprayer Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap. Analytical Biochemistry212,436-445[316L Cold Rolled Stainless Steel Coil 316L Inox]

Dalev, P.G. and Simeonova, L.S.(1992),48V DC Powered Air Conditioner for Outdoor Cabinet 2000W. Biotechnology letters 14,6, 531-534[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

Heidemann, E.(1997),New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides.Leder 48,(5),99-104[Steam Diffuser For Essential Oils]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Home Use Standing Modern Floor Lamp (KAF6108): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Home Building Materials 3D Wallpaper Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[90m3 Per Hour Mobile Concrete Pump]

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Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Frozen Soybean in Pod]

Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, Part I.Nutrition Enhancer Environmental Nutritional Yeast Powder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer]

Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine.New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, 175-184[14 Laser Welded Concrete Diamond Saw Blade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine]

Whitaker,J.R.(1963),Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase. Analytical Chemistry35(12), 1950-1953[Punching Bending Welding Steel Work Fabrication]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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