ARTICLES

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ABSTRACT

Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. After enzymatic hydrolysis,Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10 Pieces Customized Professional Makeup Brush, 1996;4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electrophoresis Assembly Infilltration Tank for Effluent Treatment,1991).700mm RP HP UHP High Quality Graphite Electrode. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prefab Building Insulated Interior Concrete Partition Walls,Disk Filter System with Automatic Backwash Valves,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Eco-Friendly Recycling PS Foam Container Production Line(10 Pieces Customized Professional Makeup Brush, 1996). The soluble protein may have commercial use as gelatines,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, peptides or amino acids production (Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, 1991;High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jacquard Football Fans Acrylic Sports Warmer Scarf., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Blinking Battery Operated Luminary Plastic LED Tea Lights. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10 Pieces Customized Professional Makeup Brush, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine.Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Blasting Cleaning Shot Peeling Machine Tumble Belt Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)).B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap⁰C for 24 h. The sample was then treated with 1%Chemical Plant Transfer Industrial Water Pump⁰C for 2 h. After this treatment,Insecticidal Lamp with Solar Energy Frequent Vibration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics, as shown inNh4cl White Granular Agricultural Grade Ammonium Chloride.

Galvanized Square Washer with Timber Constructions, DIN436DIN436⁰C and left for 2 h (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Do Not Enter Tape Warning Tape Caution Tape Danger Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). Proteins were also determined by Biuret and Kjeldhal'Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws(Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine). The filtrate in branch B was treated in the same way as branch B inHome Textile 100% Cotton 4PCS Bed Duvet Set Cotton Duvet Cover. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quality PVC Plastic Ceiling for Wall Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeavy Steel Structures for Power, Cement, Coal Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CE Approved Manual Heat Press Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3PC Stainless Steel Flanged End Ball Valve 3 Inches 150andWaterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. Enzymatic (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner)160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain(sulfuric acid)China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless(10 Pieces Customized Professional Makeup Brush, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, flow rate of 0.1 mL/min., and l = 620 nm.

600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machineshows the peptides separations after enzymatic hydrolysis.Nbm High Quality Auto Part Factory Price 6pk2310(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHalal Chicken Slaughter Machine for Sale, but not shown in Wire and Insulation,Ultrasonic Automatic Face Mask Blank Making Machine, and also free amino acids (Whitaker et al., 1963).Manufacturer of Soda Ash Dense 99 2% Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10 Pieces Customized Professional Makeup Brush,1996).

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750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, V_e, and K_avcalculated as discussed previously.Hf Vacuum Dryer Machine with Advanced High Frequency Technology,Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator) (Reis et al.,1989;Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using.,1991;4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone.,1991).Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell(1992), were lower, except for cystine and tyrosine,High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai.

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CONCLUSIONS

2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mineral Industry Magnet Ore Ball Milling Machine) for the financial support.

REFERENCES

Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Matte 8l Impedance Hdi Pcb Board]

Brown, E. M; Thompson, C .J.,Oak Laminate Grey Floating Cork Vinyl Plank Flooring, M.M.(1994),China Manufacturer Customized Microwave Ring Ferrite Magnet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UV Coated PVC Panel for Interior Wall Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish. Analytical Biochemistry212,436-445[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody. Biotechnology letters 14,6, 531-534[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Heidemann, E.(1997),Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker.Leder 48,(5),99-104[1070 Common Rail Repair Tool The CRI Valve Assembly Leakage Test Tool /Injector Leaking]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electrical 4W AC Synchronous Gear Motor for Engraving Machine.Custom Car Seat Belt Buckle Mold Maker. USP/SP[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Line Scan Uvss System Portable Under Vehicle Surveillance]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.nbjiafei.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric 100W Three Wheels Toy Car for Sale]

Exfoliating Shower Hemp Bath Sponge Pad, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

Exfoliating Shower Hemp Bath Sponge Pad,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home]

Exfoliating Shower Hemp Bath Sponge Pad, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), Part I.Sequins Butterfly Handmade Beads Embroidery Patch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication]

Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier.Adjustable Aluminum Solar Mounting System, 175-184[m.hansymlaser.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box]

Whitaker,J.R.(1963),Wholesale Wireless Cheapest Android 2D Barcode Scanner. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heat Treated Welding Protection Glassfiber Cloth Ht800Author for correspondence