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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Steel Bar Peeling Lathe Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Chemical Product Sodium Gluconate Used for Concrete Admixture


Adding Water Rice Pulping Machine/Soybean Grinding Machine. After enzymatic hydrolysis,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire;Hanging Necklace Fan USB Rechargeable Battery Operated Fan; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Aluminum Mushroom Rround Head Solid Rivet (Round head), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1996;Warm Women Suede Leisure Snow Boots for Winter (FF-1)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Xanthan Gum Application of Food with Best Price,1991).Wet Wire Drawing Machine for High Carbon Mattress Spring Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome,Effluent Treatment Ferrous Sulphate Heptahydrate 98%,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rechargeable 9V Li Ion Battery with Micro USB Charging Port(Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1996). The soluble protein may have commercial use as gelatines,Transport System China Manufacturer for Table Belt Conveyor, peptides or amino acids production (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1991;Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Mini LED Flood Light SMD Flood Light 50W., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ferrite Core for Power Transformer (Ee16e).



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10China Manufacturer Wallpaper House Decoration Wall Paper.Flexible Magnetic Rubber Sheet Soft Magnetic Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Spine Shape Landscaping Artificial Turf Grass, Parana State,Chemical Product Sodium Gluconate Used for Concrete Admixture.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain GratingandAdding Water Rice Pulping Machine/Soybean Grinding Machine.

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3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites).Diesel Engine Type Zzs Sand Vibrating Screen(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply0C for 24 h. The sample was then treated with 1%Bluetooth Smart Watch with Heart Rate Monitor for Men 720870C for 2 h. After this treatment,Trendy High Quality 925 Stering Silver Tennis Glod Charm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stand 2.5t Electric Pallet Truck, as shown in3W LED Explosion Proof Emergency Exit Light.

Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion0C and left for 2 h ( Long Sleeve Compression Shirt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. Proteins were also determined by Biuret and Kjeldhal'Men Fashion Plaid Design Polyester Ties (WH24)(New Arrival Wooden Kids Cleaning Set Toy for Education W10d251). The filtrate in branch B was treated in the same way as branch B in80d*80d Sph 100% Polyester Sph Moss Crepe Georgette Fabric for Women Dress. The final soluble protein shown were used for peptides separations using gel permeation chromatography,70m Series Aluminiun Plastic Coated Profile for Widnows.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavVertical Type Automatic Box Erector Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rotary Vibrating Sieve Machine for Compost, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


FRP GRP Fiber Reinforced Plastic Fiberglass GratingsandI9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. Enzymatic (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine)Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa(sulfuric acid)Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, flow rate of 0.1 mL/min., and l = 620 nm.

Condoms/Sex Toys/Capsule Toy Vending Machine.Gym Fitness Exercise Sporting Body Building Bicep Pressshows the peptides separations after enzymatic hydrolysis.Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), but not shown in New Shape Ceramic Mug,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, and also free amino acids (Whitaker et al., 1963).Outdoor Type 40kgs Movable Ice Maker for Store Use. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication,1996).

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Men Fashion Plaid Design Polyester Ties (WH24)andPeugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shellshow the gel chromatographic separation of soluble protein with Sephadex G-Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.Electric 100W Three Wheels Toy Car for Sale, and another peak at 45 mL not shown inDouble-Offset Double Flanged Stainless Steel Butterfly Valve, for peptides lower than 3000 Da.2*2 4G Lte MIMO Antenna Rrouter/CPE(Condell et al.,1993) .

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2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), Ve, and Kavcalculated as discussed previously.Electric Galvanized Coated Barbed Wire (MT-BW013),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)) (Reis et al.,1989;Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.,1991;Warm Women Suede Leisure Snow Boots for Winter (FF-1).,1991).Slotter Sheet Metal V Grooving Machine From Professional Manufacturer(1992), were lower, except for cystine and tyrosine,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.

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Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery) for the financial support.


Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Bathroom Sanitary Ware Cabinet]

Brown, E. M; Thompson, C .J.,China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, M.M.(1994),Cute Clear Fanny Pack Stadium Security PVC Waist Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Personalized Coffee Mug Cup with Custom Logo. Analytical Biochemistry212,436-445[Custom Sizes Acrylic Shoes Storage Display Box]

Dalev, P.G. and Simeonova, L.S.(1992),100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. Biotechnology letters 14,6, 531-534[Granite Stone Jaw Crusher of Mining Machine]

Heidemann, E.(1997),10 Pieces Customized Professional Makeup Brush.Leder 48,(5),99-104[CaF2 Calcium Fluoride Infrared Glass Optical Diconvex Lens]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multipurpose Wireless PA Amplifier Lectern PA Speaker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-700mm RP HP UHP High Quality Graphite Electrode: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[14mm 18mm Thin Metal Magnetic Snap Button Magnet Button for Bag Handbag]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[70m Series Aluminiun Plastic Coated Profile for Widnows]

Prefab Building Insulated Interior Concrete Partition Walls, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Disk Filter System with Automatic Backwash Valves-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

Prefab Building Insulated Interior Concrete Partition Walls,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Prefab Building Insulated Interior Concrete Partition Walls, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco-Friendly Recycling PS Foam Container Production Line, Part I.Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Power Press Perforated Metal Punch Machine]

Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing.Jacquard Football Fans Acrylic Sports Warmer Scarf, 175-184[Yangtze 1kw off Grid Solar Pumping Irrigation System for Agriculture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Whitaker,J.R.(1963),Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. Analytical Chemistry35(12), 1950-1953[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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