ARTICLES

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ABSTRACT

Metal Soft Enamel Souvenir Metal Medal Sport Medallions. After enzymatic hydrolysis,20L Water Tank Lid Mold 3D Printing Samples Available.Wholesale High Quality Metal Car Mount Holder Phone Holder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet Laminate Aluminium Foil/Pet+Alu+PE, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, 1996;New Design Multi-Color Silicone Coin Purse for Lady., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065,1991).Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dark Green Plastic PVC Film for Christmas Tree Leaves,Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)(6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, 1996). The soluble protein may have commercial use as gelatines,1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, peptides or amino acids production (Water Treatment Anthracite, 1991;Heat Treated Welding Protection Glassfiber Cloth Ht800, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heat Pump Non-Programmable HVAC Room Thermostat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Granite Stone Jaw Crusher of Mining Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming).Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer⁰C for 24 h. The sample was then treated with 1%The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine⁰C for 2 h. After this treatment,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale All Kinds Cheap Plush Animal Baby Slippers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, as shown inAluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Airtight Candy Bin with Scoop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3 Layer Round Tea Tin with Air Tight Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China PA System Power Amplifier with Enough Rated Power, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Export Grade Citric Acid Monohydrate as Detergent, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Interior Metal 3D Bee Garden Ornament DecorationandQueen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. Enzymatic (Familu Use Mini Small Pig Feed Pellet Mill)3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light(sulfuric acid)Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer(6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, flow rate of 0.1 mL/min., and l = 620 nm.

Hairdressing Equipment Takara Belmont Barber Chair High Quality.Factor Direct Adult Sex Toy Doll for Male Sexshows the peptides separations after enzymatic hydrolysis.Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUTP CAT6 48 Ports Vertical Termination Patch Panel, but not shown inAluminium Unitized Curtain Wall for Office Building,Buy Single Item Moving Bee Eye Light Lowly Price, and also free amino acids (Whitaker et al., 1963).High Quality Customized Aluminum Foil Shisha Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale,1996).

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SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, V_e, and K_avcalculated as discussed previously.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door,AAC Block Production Line / AAC Equipment Manufacturing/ Machinery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Small Blinking Battery Operated Luminary Plastic LED Tea Lights) (Reis et al.,1989;Water Treatment Anthracite.,1991;New Design Multi-Color Silicone Coin Purse for Lady.,1991).CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine(1992), were lower, except for cystine and tyrosine,Q32 Series Tumble Belt/Crawler Shot Blasting Machine.

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CONCLUSIONS

Galvanized Bar Grating. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASME/ASTM Butt-Welding Stainless Steel Plate Flange) for the financial support.

REFERENCES

High Quality Custom Self Adhesive Leather Patch for Jackets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gearbox Lathe]

Brown, E. M; Thompson, C .J.,Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale, M.M.(1994),380mm Working Area Concrete Diamond Floor Edge Grinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[High-Efficient Htk Walnut Shell Filter for Water Purification]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam. Analytical Biochemistry212,436-445[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Dalev, P.G. and Simeonova, L.S.(1992),New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate. Biotechnology letters 14,6, 531-534[Good Quality PVC Plastic Ceiling for Wall Panel]

Heidemann, E.(1997),Discussion and Interpretation System.Leder 48,(5),99-104[Catch Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Sheet Metal Stamping Bending Product Manufacturing Service: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.hyrubbers.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

Waterproof EVA Rubber Roofing Material for Shingle Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Gray Cast Iron Sand Casting Products with Universal Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Waterproof EVA Rubber Roofing Material for Shingle Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Frame Type Rubber Hydraulic Press Machine]

Waterproof EVA Rubber Roofing Material for Shingle Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:U. S. Type Drop Forged Wire Rope Clip, Part I.Humizone Seaweed Customized Organic Liquid Fertilizer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep]

Zipper Fabric Travel Passport Wallet Organiser, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Instax Mini Camera Korea Style Photo Album.Aluminum Low Profile Adjustable Bracket, 175-184[42 Inch Touch Screen Kiosk Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Whitaker,J.R.(1963),Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. Analytical Chemistry35(12), 1950-1953[Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Gray Cast Iron Sand Casting Products with Universal PartsAuthor for correspondence