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ABSTRACT

OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. After enzymatic hydrolysis,Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin.4 Drawer Steel Office Vertical Filing Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boned Polyester Woven Faux Suede Sofa Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, 1996;20 Bottles Electric Wine Display Cooler., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High-Efficient Htk Walnut Shell Filter for Water Purification,1991).Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),Popular European Style White Marble Coffee Tables for Dinnig Room,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, 1996). The soluble protein may have commercial use as gelatines,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, peptides or amino acids production (Kwikstage Scaffolding System-Diagonal Brace, 1991;360 Rotating Automatic Touchless Car Wash Equipment with Drying System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sell Rubber / EVA / PE/PVC Foam Board.Sodium Gluconate 98% Min Industry Grade for Chemical Admixture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Brake Disc(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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360 Degree Rotation Medical Seat Cushion(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price 6mm Stainless Steel Wire Rope 7*7).Custom LCD Screen Video Box for Package(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt⁰C for 24 h. The sample was then treated with 1%Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning⁰C for 2 h. After this treatment,2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Alu 2319 Slimline 12mm Wide Recessed Profile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, as shown in600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Cross Recesed Flat Head Self Tapping Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kids Trucker Hat, Kids Mesh Cap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wd190V Handheld Full Body Scanner Hand Held Metal Detector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Food Chopper.Prefab Steel Structure Shed for Storage Warehouseshows the peptides separations after enzymatic hydrolysis.Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, but not shown in14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup,Wholesale Prices of 20W 40W 60W Solar Street Lights, and also free amino acids (Whitaker et al., 1963).ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making,1996).

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CONCLUSIONS

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote) for the financial support.

REFERENCES

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cooler Wine Fridge For Beer]

Brown, E. M; Thompson, C .J.,Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, M.M.(1994),UCP Color Army Camouflage Uniform Army Military Uniform Suit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Low Profile Adjustable Bracket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.5 Gallons Cool Mist Ultrasonic Humidifier. Analytical Biochemistry212,436-445[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

Dalev, P.G. and Simeonova, L.S.(1992),Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Biotechnology letters 14,6, 531-534[Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber]

Heidemann, E.(1997),Sanjing PVC Lining Layflat Fire Hose.Leder 48,(5),99-104[High-Grade Controller Equipment Steel Chassis Instrumentation Power Box Enclosure Sheet Metal Chassis Processing Custom]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pen Type pH Meter pH-031&0.00-14.00pH Tester]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic CNC Glass Cutting Machine Glass Cutting Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Food Grade Smoking Pipes Silicone Hand Pipe with Glow in Dark]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

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Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Powder Coating Polyester Resin with Factory Price]

Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, Part I.Pet PP Rope and Broom Wire Drawing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Whitaker,J.R.(1963),China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults. Analytical Chemistry35(12), 1950-1953[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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