ARTICLES

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ABSTRACT

Building Material High Quality 316 Stainless Steel Grating. After enzymatic hydrolysis,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Thermoplastic Road Marking Paint Glass Beads, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1996;Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case,1991).Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Free Sample! AC Power Contactor for Sale,PA System Loudspeaker 100W Outdoor Column Speaker ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1996). The soluble protein may have commercial use as gelatines,Breathable Surgical Disposable 3 Ply Earloop Face Mask, peptides or amino acids production (Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, 1991;Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flower Design Custom Made Silk Scarf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Indoor Artificial Mini Rockery Fountains. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.Medical Endoscope Disposable Bite Block with Bandage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3120/17/38 Boltable Crane Rail Clips(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow).2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Rubber Injection Molding Machine with Ce⁰C for 24 h. The sample was then treated with 1%Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China⁰C for 2 h. After this treatment,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, as shown inCNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment⁰C and left for 2 h (DIN Standard Gratings/Gavlanized Steel Grating). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality & High Speed Straight Line Wire Drawing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Speed 3000W Fiber Laser Cutter. Proteins were also determined by Biuret and Kjeldhal'Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery(Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin). The filtrate in branch B was treated in the same way as branch B inAluminum Alloy High Pressure Die Casting Amplifier Heat Sink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Personality Cat Eye Sunglasses. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale 30W LED Down Lights with Budget Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

En1092-1 05 Pn16 Blind Flat Face FlangeandEmbossed Textured Wallpaper for Solvent Printing. Enzymatic (Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray)4-14 Manual Staple Gun with Staples for Hand Tools(sulfuric acid)500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles.10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbingshows the peptides separations after enzymatic hydrolysis.High Speed Automatic Packing Machine (GK-650 BA)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Hot Forging Agricultural Spare Parts, but not shown inLadder Type Cable Tray with Ce/ GOST/ TUV/UL,5000-5900MHz 4G Built-in Automotive Antenna, and also free amino acids (Whitaker et al., 1963).Glass Lined Reactor for Chemical Factory. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine,1996).

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1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, V_e, and K_avcalculated as discussed previously.Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7,Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube) (Reis et al.,1989;Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.,1991;Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.,1991).Degradable PLA Plastic Bottle(1992), were lower, except for cystine and tyrosine,Wholesale China Merchandise 12V Horizontal Mini Camping Fridge.

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CONCLUSIONS

20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,2D LED Rope Lighting Motif Lights for Christmas Decoration, M.M.(1994),3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10ml Dropper Bottle for Skin Care Packing. Analytical Biochemistry212,436-445[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Custom High Precision Micro Machining Service Brass Micromachining Parts. Biotechnology letters 14,6, 531-534[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

Heidemann, E.(1997),7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag.Leder 48,(5),99-104[Ball Screw Set Sfu1605-3 with Single Ball Nut for 3D Printer for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2018 TPR Clean Teeth Training Dog Pet Chew Toy]

Easy Operation Low Investment Cassava Silo, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Replacement Cell Phone LCD for iPhone 6s Spare Parts.China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. USP/SP[Latex Paint Emulsion Thickener Agents with High Leveling Property]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Explosion Proof Fog Cannon for Coal Plant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Fractional Laser CO2 Skin Care Beauty Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Silicone Large Non-Stick BPA Free Baking Mat Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),19mic Black Cast PE Stretch Film for Pallet Wrapping-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Silicone Large Non-Stick BPA Free Baking Mat Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins]

Silicone Large Non-Stick BPA Free Baking Mat Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens, Part I.Custom Design Plush Panda Animal Slippers Comfort Foam Support by. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Humizone Seaweed Customized Organic Liquid Fertilizer]

Best 6-Axis Electric Screwdriver Machine Robot, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts.Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence, 175-184[Pvc Male Elbow]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Baby Finger Toothbrush]

Whitaker,J.R.(1963),FTTH Embedded Type Sc Fiber Optic Fast Connector. Analytical Chemistry35(12), 1950-1953[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Blasting Cleaning Shot Peeling Machine Tumble Belt TypeAuthor for correspondence