ARTICLES

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ABSTRACT

Rotary Vibrating Sieve Machine for Compost. After enzymatic hydrolysis,I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Condoms/Sex Toys/Capsule Toy Vending Machine, 1996;Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price,1991).Outdoor Type 40kgs Movable Ice Maker for Store Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts,Electric 100W Three Wheels Toy Car for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2*2 4G Lte MIMO Antenna Rrouter/CPE(Condoms/Sex Toys/Capsule Toy Vending Machine, 1996). The soluble protein may have commercial use as gelatines,2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), peptides or amino acids production (Electric Galvanized Coated Barbed Wire (MT-BW013), 1991;Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Slotter Sheet Metal V Grooving Machine From Professional Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Condoms/Sex Toys/Capsule Toy Vending Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cute Clear Fanny Pack Stadium Security PVC Waist Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Prefab Building Insulated Interior Concrete Partition Walls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing).Disk Filter System with Automatic Backwash Valves(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Eco-Friendly Recycling PS Foam Container Production Line⁰C for 24 h. The sample was then treated with 1%Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve⁰C for 2 h. After this treatment,Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Jacquard Football Fans Acrylic Sports Warmer Scarf, as shown inFood Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.

Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound⁰C and left for 2 h (Aluminum Foil Heat Shield Screen Matting Adhesive Backed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Small Blinking Battery Operated Luminary Plastic LED Tea Lights. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. Proteins were also determined by Biuret and Kjeldhal'Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole(Clear PVC Sheet with Coating for Card). The filtrate in branch B was treated in the same way as branch B inHigh Intensity Fire Rated Interior Office Wood Plastic Floor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Precision Plastic Mold Manufacturer in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMango Drying Machine/Solar Fruit Drying Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.China Manufacturer Wallpaper House Decoration Wall Papershows the peptides separations after enzymatic hydrolysis.Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSquare Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, but not shown inChina Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall,Galvanized Square Washer with Timber Constructions, DIN436DIN436, and also free amino acids (Whitaker et al., 1963).Do Not Enter Tape Warning Tape Caution Tape Danger Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Condoms/Sex Toys/Capsule Toy Vending Machine,1996).

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CONCLUSIONS

High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10 Pieces Customized Professional Makeup Brush) for the financial support.

REFERENCES

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kitchen Utensils of 12PC Stainless Steel Cookware Set]

Brown, E. M; Thompson, C .J.,OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, M.M.(1994),160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Grey Full-Zip Cotton Mens Hoodies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless. Analytical Biochemistry212,436-445[24k Gold Whitening Anti-Spot Mask]

Dalev, P.G. and Simeonova, L.S.(1992),AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. Biotechnology letters 14,6, 531-534[Low-E Insulating Glass Curtain Wall]

Heidemann, E.(1997),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.Leder 48,(5),99-104[DIN7504 Roofing Screw Self-Drilling Screws for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modular Portable Shipping Container Lkit Building Home Villa House]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer of Soda Ash Dense 99 2% Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manual Vertical Car Key Cutting Machine for Locksmith]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Br38 150w Infrared Heating Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Headband Big Headphone EVA Bag Tool Case Specialized Storage Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1650mm*80mm Stable Power Laser Tube]

Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835]

Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chicken Slaughtering Machine for Sale, Part I.750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hero Brand Polypropylene Bags Machine]

Hf Vacuum Dryer Machine with Advanced High Frequency Technology, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell, 175-184[Silk Stretch Dobby Fabric Georgette Fabric]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bobby Flay Websites Restaurant Martha Stewart Designer Tray]

Whitaker,J.R.(1963),High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. Analytical Chemistry35(12), 1950-1953[Export Grade Citric Acid Monohydrate as Detergent]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1325 CNC Engraving RouterAuthor for correspondence