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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. After enzymatic hydrolysis,Day and Night Zebra Blind Fabric Z1602.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic OPP Slitter Cutting Machine (HX-320FQ vertical);Impact Sound Isolation Wall Clip; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1996;Top Quality Galvanized Iron Wire as Binding Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (13.56 MHz Fudan FM11RF08 Chip Card,1991).Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap(German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1996). The soluble protein may have commercial use as gelatines,Square Premium Glass Perfume Bottle 100ml, peptides or amino acids production (Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, 1991;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Inflatable PVC Flocking Health Bed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.Cast Iron Butterfly Valve with Handles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Furniture Grade E1 Glue Plywood with Competitive Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, Parana State,Ozonated Water for Vegetables and Fruits Washing Ozone Generator.Hexagonal Netting/ Gabion Box/ Gabion Basket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Partsand37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.

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Different Thickness High Quality Aluminum Foil for Glass Bottle Lid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper ( KTV).Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages0C for 24 h. The sample was then treated with 1%Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration0C for 2 h. After this treatment,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Ozone Disinfection Machine Ozone Generator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, as shown inCustom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet.

PVC Profile with Net Making Machine Used for Window Sealing Profile0C and left for 2 h (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Bobby Flay Websites Restaurant Martha Stewart Designer Tray. Proteins were also determined by Biuret and Kjeldhal'Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line(Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home). The filtrate in branch B was treated in the same way as branch B inMetal Logo Front. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Diameter 2.68m Paragraphed Autoclave for AAC Bricks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavFirefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Selling Household Living Room Mat Chenille Carpets, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screenand130HP 4WD Large Farm Power Engine Tractor From High Quality Factory. Enzymatic (Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment)Large Size Outdoor Store Front Signs Advertising Billboard(sulfuric acid)Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg(German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%1325 CNC Engraving Router, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%1325 CNC Engraving Router, flow rate of 0.1 mL/min., and l = 620 nm.

Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnectorshows the peptides separations after enzymatic hydrolysis.T6s 86 PCD Core Bit for Geotechnical Drilling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, but not shown inFelt Belt for Textile Compactor,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, and also free amino acids (Whitaker et al., 1963).CNC Machining 6601 Aluminum Parts with Anodized Automoblile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (German Technology Automatic Stacking System in Red Brick Automatic Production Line,1996).

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NPK 15 15 15 Compound Fertilizer at a Hot Selling, Ve, and Kavcalculated as discussed previously.Mini Blown Film Extrusion,Pearls Flake 99% Factory Naoh Caustic Soda. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing) (Reis et al.,1989;Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.,1991;Top Quality Galvanized Iron Wire as Binding Wire.,1991).Auto Spare Parts 12629037 Ignition Coil for Cadillac(1992), were lower, except for cystine and tyrosine,High Quality PVC Resin with Low Price.

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Small Backhoe Loader with Air Conditioning. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stamping Metal Parts Manufacturer Sheet Parts Fabrication) for the financial support.


Line Scan Uvss System Portable Under Vehicle Surveillance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Car Oil Hose]

Brown, E. M; Thompson, C .J.,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, M.M.(1994),FSTN Type Monochrome Graphic Display Cog LCD with Backlight.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D Embossed Iron on Label Clothing Heat Transfer Label. Analytical Biochemistry212,436-445[New Design Indoor Artificial Mini Rockery Fountains]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Food Jar & Plastic Lid. Biotechnology letters 14,6, 531-534[Galvanized Square Washer with Timber Constructions]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Derivatives]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Building Material High Quality 316 Stainless Steel Grating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Type Price Prefabricated Roof Tile in Sudan]

Building Material High Quality 316 Stainless Steel Grating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Building Material High Quality 316 Stainless Steel Grating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, Part I.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Quality Shaper Machine Bc6085 Shapping Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Thermoplastic Road Marking Paint Glass Beads.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 175-184[Aluminum Repeater Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Aluminium Windows Thermal Break China Supplier]

Whitaker,J.R.(1963),Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. Analytical Chemistry35(12), 1950-1953[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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