ARTICLES

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^IICNPq/RHAE/biotechnology-PTFE Seat Wafer Butterfly Valve Pn16. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part

ABSTRACT

China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults. After enzymatic hydrolysis,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt;Plain Makeup Bag Canvas Cosmetic Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1996;High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry,1991).Factory Wholesale Qualited Polyester Padding Men Jacket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam,Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration(1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1996). The soluble protein may have commercial use as gelatines,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, peptides or amino acids production (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1991;River Water Cleaning Sludge Sediment Machine Lamella Clarifier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polyester Coated Stainless Steel Ladder Type Metal Ties., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Copper Laminated Steel Strip T2/C11000.Halal Empty Gelatin Capsule for Medicine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, Parana State,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAnti Slip Rubber MatandSany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.

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Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence).Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price⁰C for 24 h. The sample was then treated with 1%Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine⁰C for 2 h. After this treatment,Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, as shown inFactory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile.

Plastic Food Box Container Covers by Injection Mould Mold Tool⁰C and left for 2 h (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Proteins were also determined by Biuret and Kjeldhal'Passenger Home Sightseeing Elevator Good Sales with Professional Technology(Prefab Building Insulated Interior Concrete Partition Walls). The filtrate in branch B was treated in the same way as branch B inSingle Facer Corrugated Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vacuum Stainless Steel Casting Products.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Silicone Reusable Food Storage Bag China Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polyurethane Gasket Foam Seal Dispensing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ASTM Iron Sand Casting PartsandImee Print Customized Printing Agenda Printing Nb21. Enzymatic (Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml)High Performance Multifunctional Crawler Mini Skid Steer Loader(sulfuric acid)Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chunke Portable UV Water Sterilizer for Water Purifier 150t, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chunke Portable UV Water Sterilizer for Water Purifier 150t, flow rate of 0.1 mL/min., and l = 620 nm.

Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress.3120/17/38 Boltable Crane Rail Clipsshows the peptides separations after enzymatic hydrolysis.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, but not shown inP-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails,Xjm Series Rubber Extruding Machine, and also free amino acids (Whitaker et al., 1963).Stop Dog From Jumping or Climbing Your Chain Link Fence.. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose,1996).

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Sublimation Polyester Sports Tshirt for Adult, V_e, and K_avcalculated as discussed previously.Heavy Duty Marquee Advertising Pop up Tent,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10ml Dropper Bottle for Skin Care Packing) (Reis et al.,1989;Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.,1991;High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.,1991).Fire Extinguisher UV Curing Machine Cylinder Drying Machine(1992), were lower, except for cystine and tyrosine,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set.

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CONCLUSIONS

Energy-Saving Biogas Double Stove for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Steel Frame Membrane Structure Car Parking Shed Cover Design) for the financial support.

REFERENCES

Valve Bag Dry Mortar Bagging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce Certificated 20W/50W/80W/100W/120W Outdoor All in One Integrated LED Solar Street Light with Lithium Battery & Motion Senor]

Brown, E. M; Thompson, C .J.,High Quality 500m 100lb Super Strong Braided Fishing Line, M.M.(1994),20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electrical 4W AC Synchronous Gear Motor for Engraving Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. Analytical Biochemistry212,436-445[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Biotechnology letters 14,6, 531-534[Adjustable Aluminum Solar Mounting System]

Heidemann, E.(1997),PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.Leder 48,(5),99-104[Canning Machine Tin Can Sealing Machine for Chill Sauce Tdfj-160]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stand 2.5t Electric Pallet Truck]

14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. USP/SP[Cross Stripe Sleeveless Yoga Wear]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Advertising Waterproof Self Adhesive sticker paper PP synthetic paper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Directly Selling Engine Standard Spark Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Discussion and Interpretation System]

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UV Laser Marking Machine (with Air Cooling System)]

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Logo Silicone Wristband, Rubber Wrist Bands, Part I.Custom All Kinds of Acrylic Shoe Display Racks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.Medical Supply U40 Disposable Insulin Syringe, 175-184[E355 Seamless Steel Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

Whitaker,J.R.(1963),Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine. Analytical Chemistry35(12), 1950-1953[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Copper Laminated Steel Strip T2/C11000Author for correspondence