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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (UV Laser Marking Machine (with Air Cooling System), 1996;Energy Saving Street Light E27 E40 27W LED Corn Bulb., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal,1991).Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts,High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw(UV Laser Marking Machine (with Air Cooling System), 1996). The soluble protein may have commercial use as gelatines,Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve, peptides or amino acids production (Polished Tzm Molybdenum Alloy Plate, 1991;Iron Welded Gabion Basket Retaining Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (UV Laser Marking Machine (with Air Cooling System), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰FTTX Solution Provider Fiber Optical 12cores MPO Cassette.Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Height Adjustable Cane(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop China Forklift Supplier & Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Spraying Drying Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Solar Powered Traffic Blinking Emergency Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment) for the financial support.

REFERENCES

China Manufacturer Industrial Aluminum Floor Bar Grating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[18650 PCM Protection Board with Balanced Lithium Battery Module (lws-4s30A-058)]

Brown, E. M; Thompson, C .J.,Food Grade Stainless Steel Milk Dairy Equipment for Factory, M.M.(1994),Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Amazon Best Selling Sports Ankle Support Sleeves Compression]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Height Adjustable Cane]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump. Analytical Biochemistry212,436-445[Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse]

Dalev, P.G. and Simeonova, L.S.(1992),Low Frequency 3000W 24V 230V Inverter with Battery Charger. Biotechnology letters 14,6, 531-534[Reflective Hand Hold Thermoplastic Road Marking]

Heidemann, E.(1997),Amazon Best Selling Sports Ankle Support Sleeves Compression.Leder 48,(5),99-104[Fiberglass Asphalt/Bitumen Roof Shingles for Cement and Wooden Roof]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Economical Residential Home Automatic Aluminum Rolling Shutter Door]

High Quality Spooling Pet Polyester Mylar Film for Cable Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pen Type pH Meter pH-031&0.00-14.00pH Tester.8mm Heavy Duty Nails U Shaped Galvanized Staples. USP/SP[2000mj Q Switched for Pigment Removal with Ce Certification]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASTM Iron Sand Casting Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial Activated Carbon Water Filter Industry Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat Exchanger Stainless Steel Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

200W/300W Die Casting Aluminum LED Profile Spot Effect Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproof EVA Rubber Roofing Material for Shingle Building]

200W/300W Die Casting Aluminum LED Profile Spot Effect Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brass Pump Spare Parts for Circulating Pump]

200W/300W Die Casting Aluminum LED Profile Spot Effect Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Approved Sperm Concentration Test for Sperm Density Test, Part I.Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sand Casting Parts Large Size for Industrial Use.Durable Steel Retainer Plastic Road Safety Trench Cover, 175-184[Industrial Stackable Warehouse Plastic Work Bin for Storage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Whitaker,J.R.(1963),Iron Cast Brake Drums for Trucks. Analytical Chemistry35(12), 1950-1953[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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