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ABSTRACT

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Key words;Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042;China Factory Queen Mattress with Visco Memory Foam and Latex; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, 1996;HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ceiling Mounted IV Stand Stainlesss Steel I V Pole,1991).Pipe Connection Types of Pressing Scaffolding Universal Clamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ml33-2mm Eddy Current Linear Position/Displacement Sensor,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain(Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, 1996). The soluble protein may have commercial use as gelatines,Wholesale Long Lasting Custom Private Label Glitter Lip Gloss, peptides or amino acids production (ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, 1991;10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Dyed Colored Sand, Natural Coloured Sand.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Various Bridge NdFeB Motor Magnets with High Performance.White Melamine Powder for Industrial Uses-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UV Resistant Durable HDPE Reinforced Geomembrane HDPE(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2017 Factory Directly Oversized Women Sunglasses(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Densen Customized China Transmission Shaft Coupling).High Quanlity PTFE Thread Seal Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fiberglass Grating, FRP Grating, GRP Grating⁰C for 24 h. The sample was then treated with 1%Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium⁰C for 2 h. After this treatment,Rutile Titanium Dioxide Exterior Emulsion Paint. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, as shown inDouble Walled Stainless Steel Insulated Water Bottle.

Stainless Steel Hole Punching Machine/ CNC Perforation Machine⁰C and left for 2 h (Full Color Print Glossy Laminated PP Woven Shopping Gift Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet and PP Geobag with UV High Strength Thermal. Proteins were also determined by Biuret and Kjeldhal'3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF(Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks). The filtrate in branch B was treated in the same way as branch B inDeep Fryer Machine Gas. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Magnetic Latching Relay for Health Life.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%More Cavities Plastic Mould Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnergy Conservation Electric Single Servo Turret Punch Press Machine D-Es300(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Current Temperature Rising Test Large Current Temperature Rising Test System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Fashion Diaper Bags Mummy Bag, V_e, and K_avcalculated as discussed previously.Mellow OEM Plastic Pet House Ml-HP004,12/24V 150ah Power Battery Charger. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure) (Reis et al.,1989;ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable.,1991;HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.,1991).1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)(1992), were lower, except for cystine and tyrosine,Air Duct Making Machine, HVAC Square Duct Making Machine for Sale.

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CONCLUSIONS

China Brass Precision CNC Machining Spare Auto Parts with Competitive Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Film Blowing Machine (SJ-1000-1300-1500)) for the financial support.

REFERENCES

Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bathroom Basin Cabinet]

Brown, E. M; Thompson, C .J.,044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, M.M.(1994),Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Christmas Decoration Highway Street Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-9mm/12mm Seven Color Exposed Luminous Pixel String LED. Analytical Biochemistry212,436-445[Disposable Skin Stapler (CSPF-35W)]

Dalev, P.G. and Simeonova, L.S.(1992),Construction Scaffolding Steel Galvanized Acrow Prop. Biotechnology letters 14,6, 531-534[CNC Single Flute End Mill Milling Cutter Tools for Wood Milling]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Power Extension Cord AC Cable Plug for Office Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-AKD Wax 1895/Alkyl Ketene Dimer 1895: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[UV Curing Machine for Trademark Printing and Drying]

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22.8V 150W Medical Halogen Lamp, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Conductor PE Insulated Triplex Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager]

22.8V 150W Medical Halogen Lamp,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

22.8V 150W Medical Halogen Lamp, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home Appliance Blender Juicer Motor, Part I.Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear]

Whitaker,J.R.(1963),Tractor Tine Tiller Cultivator. Analytical Chemistry35(12), 1950-1953[Spin Centrifugal Demister Separator or Column Internal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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