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ABSTRACT

50kg Soya Bean Seed PP Woven Bags Sacks. After enzymatic hydrolysis,Iron Wire Dry Type Straight Line Wire Drawing Machine.Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fully Automatic Industrial Microwave Dehydrator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), 1996;Oral Thermometer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS),1991).Wood Fireplace Double Skin Chimney Flue Pipes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material,High Sensitivity Ground Metal Detector Searching for Treasure,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminium Profile Bending Machine for Insulating Glass(Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, peptides or amino acids production (304 Thick Wall Pressure Vessel Heat Exchanger Pipe, 1991;High Quality Betaine Anhydrous with Best Price107-43-7, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Extra Heavy Wall Thickness Seamless Tubes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shrink Sleeves Labels for Packaging Labeling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IP65 Canopy LED Light Fixture 150W LED Gas Station Light.Plastic Mould Dongguan Molding Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound).Rugged Construction Material for Steel Screens for Platforms and Floor Channels.(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FKM Rubber Gasket/Rubber O Rings in China⁰C for 24 h. The sample was then treated with 1%Inclined Waste Water Treatment Lamella Clarifier⁰C for 2 h. After this treatment,33kv 100kVA Dry-Type Distribution Transformer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, as shown in23m 4 R Arms 2017 New Design Concrete Placing Boom.

5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring⁰C and left for 2 h (Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . Proteins were also determined by Biuret and Kjeldhal'China Manufacturer Export Copper Foot Tap/Valve (HFV-001)(Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim). The filtrate in branch B was treated in the same way as branch B inBgf-800 PVC Dry Laminating Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Corrugated or Smooth Surface Helix Suction Water Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Workshop Tools 7.5 Ton Electric Winch with Dual Speed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/Vand500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. Enzymatic (RFID Blocking Leather Slim Zipper Credit Card Holder Wallet)2017 New Design Vinyl 3D Stone Wallpaper Factory in China(sulfuric acid)Custom Small Paper Gift Box with Magnet Closure for E-Cigarette(Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Agricultural Mini Tiller Farm Wheel 62cc Cultivator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Agricultural Mini Tiller Farm Wheel 62cc Cultivator, flow rate of 0.1 mL/min., and l = 620 nm.

0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materialsshows the peptides separations after enzymatic hydrolysis.Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina water meter factory water flow meter vane wheel, but not shown inCustom Personalized Zinc Alloy Engraved Keychains Award Bag Charms,High Efficient Online Hot Glue Dispensing Machine for Electronic Products, and also free amino acids (Whitaker et al., 1963).High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L),1996).

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CONCLUSIONS

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles) for the financial support.

REFERENCES

Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Grate Bar]

Brown, E. M; Thompson, C .J.,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng, M.M.(1994),0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Queen Mattress with Visco Memory Foam and Latex]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1080W Most Powerful LED Flood Light Fixtures. Analytical Biochemistry212,436-445[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Dalev, P.G. and Simeonova, L.S.(1992),OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery. Biotechnology letters 14,6, 531-534[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Solar Panel PV System Mc4 Circular Connector]

Best Price Aluminium Sulphate for Water Treatment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Bucket Segment Bolt 6V0937.Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture. USP/SP[Silver Hammered Round Ball and Natural Stone Necklace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Server Rack Network Data Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Selling Baby Fashion Silicone Jelly Kids Slap Watch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Emergency Exit Light Rechargeable 220V]

Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting]

Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Aluminum Alloy Metal Stamping Processing Services]

Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable, Part I.Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbon Steel Pipe ASTM A106 A53.Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel, 175-184[Best Vga Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Whitaker,J.R.(1963),Peptides Powder Ghrp 2 for Muscle Building with Super Quality. Analytical Chemistry35(12), 1950-1953[Aerobic Fermentation Organic Fertilizer Agitator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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