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ABSTRACT

High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume. After enzymatic hydrolysis,Daytai 1550nm External Modulation Optical Transmitter 1550 -50km.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, 1996;Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment,1991).Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Aluminum Carpet Topping Stage Platform,BY60100C type metal shaping machine/hydraulic shaper machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies(New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, 1996). The soluble protein may have commercial use as gelatines,China Spplier 70W Solar Street Light with Camera Monitoring System, peptides or amino acids production (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, 1991;Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.Masturbators Adult Sex Toys Japanese Love Doll-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Powder Spray Booth for Metal Furniture, Parana State,30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu.Small Tin Box for Condoms, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4 Heads High Speed Professional Paper Cardboard Laser CutterandSmall Tin Box for Condoms.

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Small Mechanical Parts

Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board).Petrochemical Loading Platform Folding Stairs(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%64mm Double Braid UHMWPE Tugboat Mooring Rope⁰C for 24 h. The sample was then treated with 1%Rubber Soles Vulcanizing Machine/ Press Machine⁰C for 2 h. After this treatment,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, as shown in5 Liter Packed Medical Ultrasound Gel with Ce and ISO.

40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade⁰C and left for 2 h (Water Filter Cartridge PP Sediment Filter Cartridge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Forged Steel Pressure Sealed Bonnet Globe Valve. Proteins were also determined by Biuret and Kjeldhal'Stable Adjustable Monitor Mounts Folding LCD Monitor Stand(DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet). The filtrate in branch B was treated in the same way as branch B inConveyor Roller And Components. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManual Double Bridge Glass Cutting Table Machine (JFQG-2620)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Price Hg1 Self Adjustable Pipe Tank Rotator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Crawler Excavator Track Bolt 3p2274, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Livining Room Rugs Geometric Patterns Carpets 6′*9′andAutomatic CNC Centerless Lathe for Peeling Steel Round Bar. Enzymatic (High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence)Ck6150 High Precision Metal Machining Horizontal CNC Lathe(sulfuric acid)Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting(New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, flow rate of 0.1 mL/min., and l = 620 nm.

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.4.2A Type a Type C Pd QC3.0 USB Chargershows the peptides separations after enzymatic hydrolysis.Factory Custom Round Aluminum Tube 5154 5454 5652 5086(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBeautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, but not shown in19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School,Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, and also free amino acids (Whitaker et al., 1963).PVC Wood Grain Decorative Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Industrial Wheeled Wooden Shoe Display Fixtures for Shop,1996).

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CONCLUSIONS

Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike) for the financial support.

REFERENCES

En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings]

Brown, E. M; Thompson, C .J.,LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering, M.M.(1994),PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Claus Process Catalyst 4-6 mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[316L 10 Micron Sintered Stainless Steel Filter Disc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts. Analytical Biochemistry212,436-445[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Dalev, P.G. and Simeonova, L.S.(1992),Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone. Biotechnology letters 14,6, 531-534[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

Heidemann, E.(1997),China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker.Leder 48,(5),99-104[Motocycle Tires]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Resin Trap Strainer]

Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine.4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps. USP/SP[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Price Aluminium Sulphate for Water Treatment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Rubber Gumboots Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

Hot Sale Loading Truck with Cummins Engine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Density Artificial Grass Football Playground Surfaces (STO)]

Hot Sale Loading Truck with Cummins Engine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

Hot Sale Loading Truck with Cummins Engine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50kg Soya Bean Seed PP Woven Bags Sacks, Part I.Iron Wire Dry Type Straight Line Wire Drawing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Barricade Tape.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, 175-184[Hollowed-out 3D Paper Greeting Card Printing with Envelope]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb]

Whitaker,J.R.(1963),Fully Automatic Industrial Microwave Dehydrator. Analytical Chemistry35(12), 1950-1953[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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