ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. After enzymatic hydrolysis,Cute Cartoon Coin Purse Children Zipper Purse.Garment Lace Coating Machine Automatic Silicone Coating Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980);Tamper Evident Security Seals Heat Resistant Electronic Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, 1996;Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm,1991).Mooncake Pastry and Plastic Packaging Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Complete Maize Starch Wet Milling Plant with High Quality,Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Furniture Cheap Iron Stacking Banquet Hall Chairs(2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, 1996). The soluble protein may have commercial use as gelatines,Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, peptides or amino acids production (Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, 1991;High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mosquito Proof Fiberglass Fly Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:19m Mobile LED Display Billboard for Advertising Screens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, Parana State,Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2).Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory 3.6V 1800mAh NiMH Rechargeable Battery Pack AAandPickled Cucumber Production Line/Making Machine.

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Stainless Steel Expanded Mesh(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Factory 8ml UV Spider Gel Nail Care).Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lf + Hf Contactless Smart Hybrid Card⁰C for 24 h. The sample was then treated with 1%Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses⁰C for 2 h. After this treatment,LED Fairy Star Light for All Kinds of Decoration Skylike. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Linear Vibrator Screener, for Granule and Powder Selection and Classification, as shown inHome Appliance Blender Juicer Motor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEp/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Size Stone Aluminum Honeycomb Panel for Facade⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mini Freeze Dryer for Sale. GRP Gratingshows the peptides separations after enzymatic hydrolysis.5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWedding Cake Knife Serving Set Stainless Steel Acrylic Handle, but not shown inHigh Zinc Coated Galvanized Wire,Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, and also free amino acids (Whitaker et al., 1963).CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin,1996).

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DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, V_e, and K_avcalculated as discussed previously.Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile,0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Blue Silica Gel with Indicating) (Reis et al.,1989;Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks.,1991;Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels.,1991).Weiying Full Computerized Plain and Invisible Sock knitting Machine(1992), were lower, except for cystine and tyrosine,Hot Double Cereal Airtight Food Dispenser.

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CONCLUSIONS

Disposable Food Packaging Paper Soup Bowl with Lids. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing) for the financial support.

REFERENCES

500ml PP Sport Water Bottle with Ball, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flour Packaging Bag 50kg]

Brown, E. M; Thompson, C .J.,Stamping Fine Blanking Sheet Metal Fabrication Part, M.M.(1994),Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Conductor PE Insulated Triplex Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Trade Assurance Beautiful Silk Lace Closure. Analytical Biochemistry212,436-445[Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Needle Rollers for Bearings. Biotechnology letters 14,6, 531-534[Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB]

Heidemann, E.(1997),Heavy Duty Steel Warehouse Storage Pallet Rack.Leder 48,(5),99-104[Minimalism Small Portable Gold Laser Cutting Machine for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Steel Round/Square Pipe Tube Production Line]

Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. USP/SP[1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Spindle Micro CNC Lathe Ck0640]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Bulk Price Yk-11 Powder for Bodybuilding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FKM Bearing Oil Seal /Customized]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Korea National Flag Banner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head]

Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vacuum Type Single Facer Corrugator]

Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Meet All Industry Needs with Openly Design High Temperature Oven, Part I.380V 3.0HP Peristaltic Pump Swimming Pool Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove]

Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),14 Gauge Twist Galvanized Black Annealed Steel Wire.2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery, 175-184[Fiberglass Pultruded Round Tube Square Pipe FRP C Channel U Channel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Whitaker,J.R.(1963),Fully Automatic Digital Pid Temperature Controller. Analytical Chemistry35(12), 1950-1953[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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