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ABSTRACT

Cog Mono Round Display LCD Module. After enzymatic hydrolysis,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Products Suppliers Camlock Coupling Quick Couplings Female;Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3.2m Vertical Wheelchair Elevator Platform for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LC Multistage Pump Mechanical Seal (KLLC), 1996;Wedding Tent Cooling System Outdoor Event Air-Con Units., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV,1991).Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prime Gi Steel Strip Galvanized Metal Strip Price,Fireproof Aluminum Curtain Wall Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(LC Multistage Pump Mechanical Seal (KLLC), 1996). The soluble protein may have commercial use as gelatines,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, peptides or amino acids production (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 1991;Adjustable Grooming Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PE Coated Paper for Salt Sugar Sachets Paper Food Grade.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LC Multistage Pump Mechanical Seal (KLLC), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter.Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, Parana State,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LC Multistage Pump Mechanical Seal (KLLC), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLuxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)andAir Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator.

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Luxury Jewelry White Gold Plated Engagement Diamond Ring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gloss Art Paper Made Packing Package).Free Sample Cheap Price Plastic Black Underwear Hanger for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay⁰C for 24 h. The sample was then treated with 1%100% Cotton Face Mask in Guangzhou⁰C for 2 h. After this treatment,OEM High Pressure Machined Part Die Aluminum Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)AISI 430 Ba/2b Embossed Stainless Steel Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, as shown inManufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike.

Drug Delivery ABS Hospital Medical Treatment Medicine Cart⁰C and left for 2 h (Hot Sale Wood Transport Log/Timber Truck Trailers for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Proteins were also determined by Biuret and Kjeldhal'Colorful Electric English Funny Music Story Module Printing Book(Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress). The filtrate in branch B was treated in the same way as branch B inPlastic Plug. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTilt-and-Turn-with-Hidden-Hinge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0.45um Dia 47mm PVDF Filter Membrane⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commecial Use Watch Display Cabinet for Watch Store Interior Design, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminium Profile Bending Machine for Insulating GlassandSlotted Round Head Aluminum Self Tapping Screw. Enzymatic (Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike)Classic Black Color Commercial Glass Door Multi Deck Refrigerator(sulfuric acid)MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(LC Multistage Pump Mechanical Seal (KLLC), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hotbar Reflow Machine for FPC of Camera, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hotbar Reflow Machine for FPC of Camera, flow rate of 0.1 mL/min., and l = 620 nm.

Full Viton Coated Half Shaft Wafer Butterfly Valve.Speedypet Fashionable New Design Oval Pet Products Cat Toilet Boxshows the peptides separations after enzymatic hydrolysis.Claus Process Catalyst 4-6 mm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMulti Purpose Microfiber Towel Car / Home / Gift / Sport Towel, but not shown in50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation,4 Different Ball Silicone Ice Cube Maker, and also free amino acids (Whitaker et al., 1963).High Quality Washable Durable Hotel Polyester Banquet Table Cloths. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LC Multistage Pump Mechanical Seal (KLLC),1996).

As Reactive Emulsifier

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Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, V_e, and K_avcalculated as discussed previously.Aerobic Fermentation Organic Fertilizer Agitator,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor Indoor Football Soccer Goal Post Net for Kids) (Reis et al.,1989;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.,1991;Wedding Tent Cooling System Outdoor Event Air-Con Units.,1991).CS1363 a Standard UK 3 Pin Power Plug(1992), were lower, except for cystine and tyrosine,Insulation Anti Earthquake Exterior Wall Foam Board.

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CONCLUSIONS

Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink) for the financial support.

REFERENCES

Easy Soak off OEM 10ml Gel Nail Polish, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Webest Package Creative Sky Blue Special Paper Jewelry Box Custom Jewellery Box in Paper]

Brown, E. M; Thompson, C .J.,Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, M.M.(1994),Recyclable PU Leather Jewelry Pouch.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Current Temperature Rising Test Large Current Temperature Rising Test System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). Analytical Biochemistry212,436-445[TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass]

Dalev, P.G. and Simeonova, L.S.(1992),Activated Alumina Used for Catalyst Carrier in Chemical Industry. Biotechnology letters 14,6, 531-534[2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine]

Heidemann, E.(1997),High Quality 45W 50W Commercial Recessed COB LED Downlight.Leder 48,(5),99-104[m.hanyingcultural.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger]

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). USP/SP[3mm White Waterproof PVC Foam Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[7 Copper Sulphate Pentahydrate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Auto Corrugated Carton Box Paperboard Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED]

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15K High Frequency Online UPS UPS RS232/USB/Snmp Optional]

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Waterproof BNC Connector for Industry, Part I.Indoor Inkjet Media Material. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Braided PTFE Hose R14 Standard Pipe]

Steel Jacket Steam Insulation Pipeline Production Famouse in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solid Steel Hotel Safe (G-42BD classics) for Hotel.Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, 175-184[5 Gallon Pc Bottle Blow Moulding Machine Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck]

Whitaker,J.R.(1963),CNC Aluminum Alloy Metal Stamping Processing Services. Analytical Chemistry35(12), 1950-1953[Daytai 1550nm External Modulation Optical Transmitter 1550 -50km]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Industrial Grade Anhydrous Sodium SulfateAuthor for correspondence