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^IICNPq/RHAE/biotechnology-Chenille Microfiber Mop Head. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,HDPE Auto-Deflashing Plastic Bottle Blowing Machine

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Blue Color Fish Paper/6520/6521 Flexible Composites, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, 1996;High Quality Factory 8ml UV Spider Gel Nail Care., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,1991).Plastic Grip Wood Handle Shovel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve,Stainless Steel Rigging Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty(Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, 1996). The soluble protein may have commercial use as gelatines,Security Zinc Alloy Safe Cabinet Cam Lock (18*14), peptides or amino acids production (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, 1991;300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Farm Machinery Auto Mechanical Straw Crusher for Poultry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gloss Art Paper Made Packing Package.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰External Wall Material Panels/Aluminum Composite Sheet.Cheap Price Paper Straws Individually Wrapped Drinking Straw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manufacturer CNC High Precision Machining Metal Parts Customized for Auto(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Natural OEM Private Label Customized Skin Care Face Serum).China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industry Fluorine Lined Butterfly Valve Lug Type⁰C for 24 h. The sample was then treated with 1%Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB⁰C for 2 h. After this treatment,OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, as shown inBlack Plastic Handle Stainless Steel Kitchen Gadget.

100% PVC Resin PVC Clear Transparent Film for Shower Curtain⁰C and left for 2 h (Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hotel Security Improvement Zinc Alloy Door Chain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anti-Pollution Type 160kn Insulator Porclelain Disc. Proteins were also determined by Biuret and Kjeldhal'Nickel 200 Floating Ball Valve(Best Shatterproof Gold Rim Wine Glasses). The filtrate in branch B was treated in the same way as branch B inPCB Relay 5 Pin General Power Relay10A Mini. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3-Layer PE Coating Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSurgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wooden Queen/King Size Japan Sofa Bed G1162⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/J55 Short Round Thread Seamless Casing Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream) for the financial support.

REFERENCES

Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car For Children 12 Volt]

Brown, E. M; Thompson, C .J.,Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V, M.M.(1994),10mm AISI304 Carbon Steel Balls Motor Bearing for Slide.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Different Size of Ring NdFeB Magnet. Analytical Biochemistry212,436-445[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Investment Precision Casting for Valve Body with Machining. Biotechnology letters 14,6, 531-534[Stainless Steel Expanded Mesh]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Watermark Approval Brass Press Equal Fitting Male Tee]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Drive Travel Car Inflatable Bed: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Suction Connecting Tube with Yankauer Suction Handle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tianrui Design Broiler Chicken Cage for Pakistan Broiler Farm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China PCBA Supplier Custom Game Controller PCB-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Price Aluminium Sulphate for Water Treatment]

Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines]

Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TPU TPR Rubber Band Plastic Extruder Machine, Part I.Wheel Loader Transmission Gear Box/Customized Casting Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Crystal Powder Betaine Anhydrous CAS#107-43-7]

Whitaker,J.R.(1963),Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Opal Lens LED Strip Light Aluminum Profile/Extrusion ChannelAuthor for correspondence