ARTICLES

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ABSTRACT

Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. After enzymatic hydrolysis,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet.3-Way T Type Elbow Quick Connect Pneumatic Fitting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Superior Quality Soya Protein Food Production Machine Line;Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Army Style 100% Polyester Fiber Mens Polo Shirt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, 1996;Twisted Annealed Wire for Binding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid,1991).Wholesale Fashion Jewellery Lady Eardrop Earrings for Women. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640,Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Automatic AAC Concrete Lightweight Block Making Machine(E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, peptides or amino acids production (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, 1991;China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Low Volume Production Aluminum CNC Machining Auto Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbon Steel Round/Square Pipe Tube Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable).Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station⁰C for 24 h. The sample was then treated with 1%off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw⁰C for 2 h. After this treatment,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, as shown inLED Emergency Exit Light Rechargeable 220V.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRide on Toys Riding Animals in The Mall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Coated Corrugated Galvanized Roofing Steel Plate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.14 Gauge Twist Galvanized Black Annealed Steel Wireshows the peptides separations after enzymatic hydrolysis.Retractable Multi Core Cable Reel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAerobic Fermentation Organic Fertilizer Agitator, but not shown inHigh Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40,New Xcm 120 Asphalt Mixing Plant for Sale, and also free amino acids (Whitaker et al., 1963).Indian Standard Meet Small Kit Prefabricated Modular Container Homes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator,1996).

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Graphic 240X128 Dots Monochrome LCD Display Module, V_e, and K_avcalculated as discussed previously.Dry & Wet Concrete Surface Metal Grinding Wing,Workbench Anti-Static Table ESD Natural Rubber Table Mat. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist) (Reis et al.,1989;Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.,1991;Twisted Annealed Wire for Binding.,1991).316L 10 Micron Sintered Stainless Steel Filter Disc(1992), were lower, except for cystine and tyrosine,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.

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CONCLUSIONS

Abstract Light and Shadow Metal Oil Painting for Deco. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Canvas School College Backpack/Bookbags for Girls/Students/Women) for the financial support.

REFERENCES

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Type J K Mineral Insulated Thermocouple Cable]

Brown, E. M; Thompson, C .J.,Cheapest Container Living House/Office/Kiosk, M.M.(1994),Spin Centrifugal Demister Separator or Column Internal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hydraulic Fold-Bend Machine Hydraulic Bending Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Slotted Round Head Aluminum Self Tapping Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Baby Wet Wipe Machine. Analytical Biochemistry212,436-445[24K Gold Rolling Paper Shine Papers Cigarette Paper]

Dalev, P.G. and Simeonova, L.S.(1992),Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. Biotechnology letters 14,6, 531-534[Ferrous Sulphate Monohydrate 30%Min Factory Wholesale]

Heidemann, E.(1997),Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric.Leder 48,(5),99-104[Safely Pass Customs Tb500 Thymosin Beta for Body Building]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4K Giant Whiteboard Interactive Panel for University Whiteboard]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Die Aluminum Castings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[European Classical Fabric Sofa Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bending Shrapnel]

High Quality Meat Pie Making Machine with Different Mould Shape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gear Operated Flanged High Performance Butterfly Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

High Quality Meat Pie Making Machine with Different Mould Shape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

High Quality Meat Pie Making Machine with Different Mould Shape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, Part I.Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Wholesale Universal Garden Smart Flower Pot, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reflective Tape for Vehicle Reflective Markings (tape-02).White Crystal Powder Betaine Anhydrous CAS#107-43-7, 175-184[Aluminum Milling Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Whitaker,J.R.(1963),Creative New Product Coin Holder Keychain. Analytical Chemistry35(12), 1950-1953[5 Gallon Pure Water Bottle Non-Spill Cap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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