ARTICLES

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ABSTRACT

Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. After enzymatic hydrolysis,Australia Two Pins Power Cord with SAA Certification.87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%;Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, 1996;Pickled Cucumber Production Line/Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rectangular Lunch Tin Box with Handle,1991).Inflatable Cheering Stick for Promotional Gift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2(Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, 1996). The soluble protein may have commercial use as gelatines,Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, peptides or amino acids production (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1991;Mini Outdoor Hydraulic Man Aluminum Lift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Price Roof Wall PU Sandwich Panel Press., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-industrial aluminum foil roll 8011 8079 1235. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Color Print Glossy Laminated PP Woven Shopping Gift Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Better Quality White Coated Fbb Paper Ivory Board).High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Quality Assurance Advanced Design Microfiber Filling Washable Pillow⁰C for 24 h. The sample was then treated with 1%Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price⁰C for 2 h. After this treatment,Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wall Mounting Metal Control Box Control Desk's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision Tooling Grinding Machine Part, as shown in4lz-4.5 Small Tank Kubota Similar Rice Harvester.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2017 Hot Diped Galvanized Welded Gabion Basket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100%Rayon Red Satin Dyeing Fabric for Women Dress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Density Artificial Grass Football Playground Surfaces (STO), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Industrial Grade Anhydrous Sodium Sulfate.Premium quality for Potassium Humate powdershows the peptides separations after enzymatic hydrolysis.Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHydraulic Cutter Bread Machine (ZMF-20Y), but not shown in100%Rayon Red Satin Dyeing Fabric for Women Dress,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, and also free amino acids (Whitaker et al., 1963).Low Cost Prefab Steel Poultry Chicken House on Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml,1996).

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CONCLUSIONS

Security Zinc Alloy Safe Cabinet Cam Lock (18*14). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Wall Glass Coffee Mug Tea Cups) for the financial support.

REFERENCES

Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Height Semi Vertical Island Half Multideck Showcase Chiller]

Brown, E. M; Thompson, C .J.,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, M.M.(1994),Farm Machinery Auto Mechanical Straw Crusher for Poultry.The Journal of the American Leather Chemists Association (JALCA)89(7)[Transmission Ultrasound Gel /20ml]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gloss Art Paper Made Packing Package. Analytical Biochemistry212,436-445[30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care]

Dalev, P.G. and Simeonova, L.S.(1992),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. Biotechnology letters 14,6, 531-534[Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins]

Heidemann, E.(1997),External Wall Material Panels/Aluminum Composite Sheet.Leder 48,(5),99-104[Aluminum Extrusion]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[U. S. Type Clevis Grab Hook]

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheap Price Paper Straws Individually Wrapped Drinking Straw.Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. USP/SP[Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mellow OEM Plastic Pet House Ml-HP004]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Cable Assembly Wire Harness Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.joinhome-sales.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers]

Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

Hot Sale Wood Transport Log/Timber Truck Trailers for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Geotextile for Roofs]

Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, Part I.China Hebei Phosphating Black Heading Machine Drywall Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Freqency PCB with Blind Via]

Strong Rare Earth Permanent Wind Driven Generator Magnets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturer CNC High Precision Machining Metal Parts Customized for Auto.China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, 175-184[Cooler Jug]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China PCBA Supplier Custom Game Controller PCB]

Whitaker,J.R.(1963),Industry Fluorine Lined Butterfly Valve Lug Type. Analytical Chemistry35(12), 1950-1953[Luxious Hotel Passenger Elevator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PPR PVC Polyurethane Insulated Pipe Extrusion Production MachineAuthor for correspondence