ARTICLES

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^IICNPq/RHAE/biotechnology-Super Potassium Humate Shiny Flakes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4K Giant Whiteboard Interactive Panel for University Whiteboard

ABSTRACT

Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. After enzymatic hydrolysis,Electric Massage Bed Pedicure Chair with Double Dust Collector.750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Adjustable Solar Mounting Kits (GD1059);Bgf-800 BOPP Dry Laminating Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Test-630 Automatic Protective Relay Tester, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, 1996;Tpdl Belt Press Sludge Equipment with Slaughtering Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS),1991).The Most Demanded Products Fluorescence Microscope Camera. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Price Promotion Soda Ash Light to Papermaking,Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HOWO 420HP Rhd Tractor Truck Head(Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, 1996). The soluble protein may have commercial use as gelatines,Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, peptides or amino acids production (Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, 1991;Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gaint Inflatable Animal Slide.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White 4*8FT 18mm PVC Foam Board for Cabinets Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Power Plant Circulating Water Pump & Spare Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Steel Stacking Restaurant Wedding Chair, Parana State,4K Giant Whiteboard Interactive Panel for University Whiteboard.Rutile Anatase Titanium Dioxide for Coating and Paints, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty EquipmentandNonwovenface Mask Production Line.

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125mm Wet Diamond Pad for Polishing Marble⁰C and left for 2 h (750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,40W Integrated Solar Street Lighting LED for Park. Proteins were also determined by Biuret and Kjeldhal'Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain(Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass). The filtrate in branch B was treated in the same way as branch B inHotel Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Design Many Styles Fancy Wine Display Rack (6532).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Checkered Plate 304 316L 410 Floor Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Sesame Bean Seed Cleaner and GraderandSport Anti Vibration Hiking Safety Shoes. Enzymatic (Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial)Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine(sulfuric acid)24W 400W Rainproof Switch Power Adapter for LED Light(Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industrial Threaded Plastic Connector Female Electrical Plug, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industrial Threaded Plastic Connector Female Electrical Plug, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile) for the financial support.

REFERENCES

Mooncake Pastry and Plastic Packaging Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[304/304L 2b Stainless Steel Sheet and Steel Plate]

Brown, E. M; Thompson, C .J.,Complete Maize Starch Wet Milling Plant with High Quality, M.M.(1994),Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole.The Journal of the American Leather Chemists Association (JALCA)89(7)[Educational Equipment Smart Multi Touch Interactive White Board for School]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED UFO Highbay 250W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Furniture Cheap Iron Stacking Banquet Hall Chairs. Analytical Biochemistry212,436-445[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. Biotechnology letters 14,6, 531-534[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Heidemann, E.(1997),Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks.Leder 48,(5),99-104[Fire Pump Unit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Belt Conveyor in Heavy Duty Industry for Mining]

Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. USP/SP[Solar Aluminum Rail Splice Connector for Rail Extention]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Document Storage Durable Metal Wire Mesh Decking for Pallet Rack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Disperse Dyes High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glass Disc Suspension Insulators]

Mosquito Proof Fiberglass Fly Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

Mosquito Proof Fiberglass Fly Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Mosquito Proof Fiberglass Fly Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board, Part I.19m Mobile LED Display Billboard for Advertising Screens. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Replica Car Wheels with 20inch for BMW Car.SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, 175-184[High Quality Gold-Plated 20pin Displayport Cable 1.3]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non-Ionic Polyacrylamide Flocculant]

Whitaker,J.R.(1963),Cooling Tower Replaced Fill Media. Analytical Chemistry35(12), 1950-1953[Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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